| Title: | Immunolocalization of podoplanin/E11/gp38, CD44, and endomucin in the odontoblastic cell layer of murine tooth germs |
| Authors: | Khadiza, Naznin Browse this author |
| Hasegawa, Tomoka Browse this author →KAKEN DB |
| Nagai, Tomoya Browse this author |
| Yamamoto, Tomomaya Browse this author |
| Miyamoto-Takasaki, Yukina Browse this author |
| Hongo, Hiromi Browse this author →KAKEN DB |
| Abe, Miki Browse this author |
| Haraguchi, Mai Browse this author |
| Yamamoto, Tsuneyuki Browse this author →KAKEN DB |
| Yi, Min Browse this author →KAKEN DB |
| Qiu, Zixuan Browse this author |
| Sasaki, Muneteru Browse this author |
| Kuroshima, Shinichiro Browse this author |
| Ohshima, Hayato Browse this author |
| Luiz de Freitas, Paulo Henrique Browse this author |
| Li, Minqi Browse this author |
| Yawaka, Yasutaka Browse this author →KAKEN DB |
| Amizuka, Norio Browse this author →KAKEN DB |
| Issue Date: | 1-Aug-2019 |
| Publisher: | Biomedical Research Press |
| Journal Title: | Biomedical Research (Tokyo) |
| Volume: | 40 |
| Issue: | 4 |
| Start Page: | 133 |
| End Page: | 143 |
| Publisher DOI: | 10.2220/biomedres.40.133 |
| Abstract: | In this study, we attempted to localize the immunoreactivities of podoplanin/E11/gp38 and CD44, a counterpart possessing a high affinity to podoplanin/E11/gp38, as well as endomucin-immunoreactive blood vessels in the regions of odontoblast layers and the underlying sub-odontoblastic layers in murine tooth germs. Endomucin-reactive small blood vessels were scattered throughout the dental papillae of the tooth germs at postnatal day 1 but came to be localized close to the odontoblast/sub-odontoblastic layers until day 3. After postnatal day 5, small blood vessels were seen in odontoblast cell layers, while blood vessels with relatively larger diameters were seen forming in sub-odontoblastic layers. Immunoreactivities of podoplanin/E11/gp38 and CD44 were not detectable in the cells of dental papillae facing the inner enamel epithelium at postnatal day 1. However, at around postnatal days 3-5, podoplanin/E11/gp38 was localized in the odontoblast layer but not in the sub-odontoblastic layer, whereas CD44 was observed in the sub-odontoblastic layer but not in the odontoblast layer. The exclusive immunolocalization of podoplanin/E11/gp38 and CD44 in the odontoblast layers and sub-odontoblastic layers was seen after postnatal day 3 of the tooth germs, when the mesenchymal cells of dental papillae have already differentiated into mature odontoblasts at the cusp tip. Taken together, it seems likely that endomucin-reactive small blood vessels extended to the podoplanin/E11/gp38-positive odontoblast layers, whereas endomucin-reactive large blood vessels were already present in CD44-immmunopositive sub-odontoblastic layer, indicating the cellular regulation on the vascularization of endomucin-reactive endothelial cells during odontogenesis of the tooth germs. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75445 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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