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Immunolocalization of podoplanin/E11/gp38, CD44, and endomucin in the odontoblastic cell layer of murine tooth germs

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Title: Immunolocalization of podoplanin/E11/gp38, CD44, and endomucin in the odontoblastic cell layer of murine tooth germs
Authors: Khadiza, Naznin Browse this author
Hasegawa, Tomoka Browse this author →KAKEN DB
Nagai, Tomoya Browse this author
Yamamoto, Tomomaya Browse this author
Miyamoto-Takasaki, Yukina Browse this author
Hongo, Hiromi Browse this author →KAKEN DB
Abe, Miki Browse this author
Haraguchi, Mai Browse this author
Yamamoto, Tsuneyuki Browse this author →KAKEN DB
Yi, Min Browse this author →KAKEN DB
Qiu, Zixuan Browse this author
Sasaki, Muneteru Browse this author
Kuroshima, Shinichiro Browse this author
Ohshima, Hayato Browse this author
Luiz de Freitas, Paulo Henrique Browse this author
Li, Minqi Browse this author
Yawaka, Yasutaka Browse this author →KAKEN DB
Amizuka, Norio Browse this author →KAKEN DB
Issue Date: 1-Aug-2019
Publisher: Biomedical Research Press
Journal Title: Biomedical Research (Tokyo)
Volume: 40
Issue: 4
Start Page: 133
End Page: 143
Publisher DOI: 10.2220/biomedres.40.133
Abstract: In this study, we attempted to localize the immunoreactivities of podoplanin/E11/gp38 and CD44, a counterpart possessing a high affinity to podoplanin/E11/gp38, as well as endomucin-immunoreactive blood vessels in the regions of odontoblast layers and the underlying sub-odontoblastic layers in murine tooth germs. Endomucin-reactive small blood vessels were scattered throughout the dental papillae of the tooth germs at postnatal day 1 but came to be localized close to the odontoblast/sub-odontoblastic layers until day 3. After postnatal day 5, small blood vessels were seen in odontoblast cell layers, while blood vessels with relatively larger diameters were seen forming in sub-odontoblastic layers. Immunoreactivities of podoplanin/E11/gp38 and CD44 were not detectable in the cells of dental papillae facing the inner enamel epithelium at postnatal day 1. However, at around postnatal days 3-5, podoplanin/E11/gp38 was localized in the odontoblast layer but not in the sub-odontoblastic layer, whereas CD44 was observed in the sub-odontoblastic layer but not in the odontoblast layer. The exclusive immunolocalization of podoplanin/E11/gp38 and CD44 in the odontoblast layers and sub-odontoblastic layers was seen after postnatal day 3 of the tooth germs, when the mesenchymal cells of dental papillae have already differentiated into mature odontoblasts at the cusp tip. Taken together, it seems likely that endomucin-reactive small blood vessels extended to the podoplanin/E11/gp38-positive odontoblast layers, whereas endomucin-reactive large blood vessels were already present in CD44-immmunopositive sub-odontoblastic layer, indicating the cellular regulation on the vascularization of endomucin-reactive endothelial cells during odontogenesis of the tooth germs.
Type: article
URI: http://hdl.handle.net/2115/75445
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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