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Identification of RNA aptamer which specifically interacts with PtdIns(3)P
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| Title: | Identification of RNA aptamer which specifically interacts with PtdIns(3)P |
| Authors: | Donia, Thoria Browse this author | | Jyoti, Bala Browse this author | | Suizu, Futoshi Browse this author →KAKEN DB | | Hirata, Noriyuki Browse this author | | Tanaka, Tsutomu Browse this author | | Ishigaki, Satoko Browse this author | | Thomas, Pranzatelli J. F. Browse this author | | Nio-Kobayashi, Junko Browse this author →KAKEN DB | | Iwanaga, Toshihiko Browse this author →KAKEN DB | | Chiorini, John A. Browse this author | | Noguchi, Masayuki Browse this author →KAKEN DB |
| Keywords: | Phosphoinositide | | RNA aptamer | | Autophagy | | Molecular imaging | | PI3K | | Lysosome |
| Issue Date: | 10-Sep-2019 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and biophysical research communications |
| Volume: | 517 |
| Issue: | 1 |
| Start Page: | 146 |
| End Page: | 154 |
| Publisher DOI: | 10.1016/j.bbrc.2019.07.034 |
| PMID: | 31351587 |
| Abstract: | The phosphinositide Ptdlns(3)P plays an important role in autophagy; however, the detailed mechanism of its activity remains unclear. Here, we used a Systematic Evolution of Ligands by EXponential enrichment (SELEX) screening approach to identify an RNA aptamer of 40 nucleotides that specifically recognizes and binds to intracellular lysosomal Ptdlns(3)P. Binding occurs in a magnesium concentration- and pH-dependent manner, and consequently inhibits autophagy as determined by LC3II/I conversion, p62 degradation, formation of LC3 puncta, and lysosomal accumulation of Phafin2. These effects in turn inhibited lysosomal acidification, and the subsequent hydrolytic activity of cathepsin D following induction of autophagy. Given the essential role of Ptdlns(3)P as a key targeting molecule for autophagy induction, identification of this novel Ptdlns(3)P RNA aptamer provides new opportunities for investigating the biological functions and mechanisms of phosphoinositides. (C) 2019 The Authors. Published by Elsevier Inc. |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75448 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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