|
Hokkaido University Collection of Scholarly and Academic Papers >
Global Institution for Collaborative Research and Education : GI-CoRE >
Peer-reviewed Journal Articles, etc >
Feasibility and efficiency of human bone marrow stromal cell culture with allogeneic platelet lysate-supplementation for cell therapy against stroke
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Feasibility and efficiency of human bone marrow stromal cell culture with allogeneic platelet lysate-supplementation for cell therapy against stroke |
| Authors: | Tan, Chengbo Browse this author | | Shichinohe, Hideo Browse this author →KAKEN DB | | Wang, Zifeng Browse this author | | Hamauchi, Shuji Browse this author | | Abumiya, Takeo Browse this author →KAKEN DB | | Nakayama, Naoki Browse this author →KAKEN DB | | Kazumata, Ken Browse this author →KAKEN DB | | Ito, Tsuneo Browse this author | | Kudo, Kohsuke Browse this author →KAKEN DB | | Takamoto, Shigeru Browse this author | | Houkin, Kiyohiro Browse this author →KAKEN DB |
| Issue Date: | 2016 |
| Publisher: | Hindawi |
| Journal Title: | Stem Cells International |
| Volume: | 2016 |
| Start Page: | 6104780 |
| Publisher DOI: | 10.1155/2016/6104780 |
| PMID: | 27840648 |
| Abstract: | Currently, there is increasing interest in human bone marrow stromal cells (hBMSCs) as regeneration therapy against cerebral stroke. The aim of the present study was to evaluate the feasibility and validity of hBMSC cultures with allogeneic platelet lysates (PLs). Platelet concentrates (PC) were harvested from healthy volunteers and made into single donor-derived PL (sPL). The PL mixtures (mPL) were made from three different sPL. Some growth factors and platelet cell surface antigens were detected by enzyme-linked immunosorbent assay (ELISA). The hBMSCs cultured with 10% PL were analyzed for their proliferative potential, surface markers, and karyotypes. The cells were incubated with superparamagnetic iron oxide (SPIO) agents and injected into a pig brain. MRI and histological analysis were performed. Consequently, nine lots of sPL and three mPL were prepared. ELISA analysis showed that PL contained adequate growth factors and a particle of platelet surface antigens. Cell proliferation capacity of PLs was equivalent to or higher than that of fetal calf serum (FCS). No contradiction in cell surface markers and no chromosomal aberrations were found. The MRI detected the distribution of SPIO-labeled hBMSCs in the pig brain. In summary, the hBMSCs cultured with allogeneic PL are suitable for cell therapy against stroke. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75484 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
