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Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes
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| Title: | Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes |
| Authors: | Homan, Kentaro Browse this author →KAKEN DB | | Hanamatsu, Hisatoshi Browse this author →KAKEN DB | | Furukawa, Jun-ichi Browse this author →KAKEN DB | | Okada, Kazue Browse this author | | Yokota, Ikuko Browse this author | | Onodera, Tomohiro Browse this author →KAKEN DB | | Iwasaki, Norimasa Browse this author →KAKEN DB |
| Keywords: | chondrocyte hypertrophy | | glycomics | | glycoconjugate | | glycosphingolipid | | glycosaminoglycan |
| Issue Date: | 2-Jul-2019 |
| Publisher: | MDPI |
| Journal Title: | International Journal of Molecular Sciences |
| Volume: | 20 |
| Issue: | 14 |
| Start Page: | 3546 |
| Publisher DOI: | 10.3390/ijms20143546 |
| PMID: | 31331074 |
| Abstract: | In normal articular cartilage, chondrocytes do not readily proliferate or terminally differentiate, and exhibit a low level of metabolism. Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear. Glycans are located on the outermost cell surface. Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins. This study aimed to clarify the alterations in glycans upon late differentiation of chondrocytes, during which hypertrophy-like changes occur. Primary mouse chondrocytes were differentiated using an insulin-induced chondro-osteogenic differentiation model. Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation. The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed. Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation. In addition, expression of genes related to glycan biosynthesis and metabolic processes was significantly correlated with glycan alterations. Our results indicate that total cellular glycan alterations are closely associated with chondrocyte hypertrophy and help to describe the glycophenotype by chondrocytes and their hypertrophic differentiation. our results will assist the identification of diagnostic and differentiation biomarkers in the future. |
| Rights: | © 2019 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). | | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75510 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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