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CRISPRa-mediated NEAT1 lncRNA upregulation induces formation of intact paraspeckles

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Title: CRISPRa-mediated NEAT1 lncRNA upregulation induces formation of intact paraspeckles
Authors: Yamazaki, Tomohiro Browse this author
Fujikawa, Chikako Browse this author
Kubota, Ayaka Browse this author
Takahashi, Akinari Browse this author
Hirose, Tetsuro Browse this author →KAKEN DB
Keywords: CRISPRa
dCas9
Long noncoding RNA
NEAT1
Paraspeckle
Nuclear body
Issue Date: 26-Sep-2018
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 504
Issue: 1
Start Page: 218
End Page: 224
Publisher DOI: 10.1016/j.bbrc.2018.08.158
Abstract: Long noncoding RNAs (lncRNAs) are fundamental genomic regulatory factors under various physiological and pathological conditions. A class of lncRNAs termed architectural RNAs (arcRNAs) plays an essential scaffolding role in building nuclear bodies. NEAT1 arcRNA is an abundant, nuclear-retained lncRNA that constructs paraspeckle nuclear bodies. NEAT1 is upregulated in various developmental and disease conditions including cancer and virus infection. However, it remains unclear how elevated expression of NEAT1 influences such conditions. Here, we set up an experimental method to selectively increase NEAT1 expression. We applied the synergistic activation mediator (SAM) system using catalytically dead Cas9 (dCas9) proteins to activate transcription of the NEAT1 gene. We examined 10 pre-designed and 15 originally designed single-guide RNAs (sgRNAs) in the NEAT1 promoter region for CRISPR activation (CRISPRa). We validated several sgRNAs that we designed for the SAM system to strongly activate NEAT1 expression in two human cell lines and induced formation of paraspeckles with intact core-shell structures. Thus, this selective NEAT1 upregulation method using the SAM system would be useful for further functional analyses of NEAT1 lncRNA in both basic and applied research.
Rights: © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/75519
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 廣瀬 哲郎

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