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Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations

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Title: Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations
Authors: Abdallah, M. Abdallah Browse this author
Hill-Cawthorne, A. Grant Browse this author
Otto, D. Thomas Browse this author
Coll, Francesc Browse this author
Guerra-Assunção, José Afonso Browse this author
Gao, Ge Browse this author
Naeem, Raeece Browse this author
Ansari, Hifzur Browse this author
Malas, B. Tareq Browse this author
Adroub, A. Sabir Browse this author
Verboom, Theo Browse this author
Ummels, Roy Browse this author
Zhang, Huoming Browse this author
Panigrahi, Kumar Aswini Browse this author
McNerney, Ruth Browse this author
Brosch, Roland Browse this author
Clark, G. Taane Browse this author
Behr, A. Marcel Browse this author
Bitter, Wilbert Browse this author
Pain, Arnab Browse this author
Issue Date: 21-Oct-2015
Publisher: Nature Publishing Group
Journal Title: Scientific Reports
Volume: 5
Start Page: 15443
Publisher DOI: 10.1038/srep15443
PMID: 26487098
Abstract: Although Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor contributing to this variability may be the diversity of the BCG strains that are used around the world, in part from genomic changes accumulated during vaccine production and their resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression of proteins across five representative BCG strains of the four tandem duplication (DU) groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and insertions and deletions (indels) across fourteen BCG strains. Genome-wide SNP characterization allowed the construction of a new and robust phylogenic genealogy of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may be reflected by altered immunogenicity and possibly vaccine efficacy. Together, these integrated-omic data represent the most comprehensive catalogue of genetic variation across a global collection of BCG strains.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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