| Title: | Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations |
| Authors: | Abdallah, M. Abdallah Browse this author |
| Hill-Cawthorne, A. Grant Browse this author |
| Otto, D. Thomas Browse this author |
| Coll, Francesc Browse this author |
| Guerra-Assunção, José Afonso Browse this author |
| Gao, Ge Browse this author |
| Naeem, Raeece Browse this author |
| Ansari, Hifzur Browse this author |
| Malas, B. Tareq Browse this author |
| Adroub, A. Sabir Browse this author |
| Verboom, Theo Browse this author |
| Ummels, Roy Browse this author |
| Zhang, Huoming Browse this author |
| Panigrahi, Kumar Aswini Browse this author |
| McNerney, Ruth Browse this author |
| Brosch, Roland Browse this author |
| Clark, G. Taane Browse this author |
| Behr, A. Marcel Browse this author |
| Bitter, Wilbert Browse this author |
| Pain, Arnab Browse this author |
| Issue Date: | 21-Oct-2015 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific Reports |
| Volume: | 5 |
| Start Page: | 15443 |
| Publisher DOI: | 10.1038/srep15443 |
| PMID: | 26487098 |
| Abstract: | Although Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor contributing to this variability may be the diversity of the BCG strains that are used around the world, in part from genomic changes accumulated during vaccine production and their resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression of proteins across five representative BCG strains of the four tandem duplication (DU) groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and insertions and deletions (indels) across fourteen BCG strains. Genome-wide SNP characterization allowed the construction of a new and robust phylogenic genealogy of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may be reflected by altered immunogenicity and possibly vaccine efficacy. Together, these integrated-omic data represent the most comprehensive catalogue of genetic variation across a global collection of BCG strains. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75549 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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