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Molecular epidemiology of pathogenic Leptospira spp. in the straw-colored fruit bat (Eidolon helvum) migrating to Zambia from the Democratic Republic of Congo

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Title: Molecular epidemiology of pathogenic Leptospira spp. in the straw-colored fruit bat (Eidolon helvum) migrating to Zambia from the Democratic Republic of Congo
Authors: Ogawa, Hirohito Browse this author →KAKEN DB
Koizumi, Nobuo Browse this author →KAKEN DB
Ohnuma, Aiko Browse this author
Mutemwa, Alisheke Browse this author
Hang'ombe, M. Bernard Browse this author
Mweene, S. Aaron Browse this author
Takada, Ayato Browse this author →KAKEN DB
Sugimoto, Chihiro Browse this author →KAKEN DB
Suzuki, Yasuhiko Browse this author →KAKEN DB
Kida, Hiroshi Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: Leptospira
Fruit bat
Eidolon helvum
Zambia
Pathogenic
Issue Date: Jun-2015
Publisher: Elsevier
Journal Title: Infection, Genetics and Evolution
Volume: 32
Start Page: 143
End Page: 147
Publisher DOI: 10.1016/j.meegid.2015.03.013
PMID: 25791930
Abstract: The role played by bats as a potential source of transmission of Leptospira spp. to humans is poorly understood, despite various pathogenic Leptospira spp. being identified in these mammals. Here, we investigated the prevalence and diversity of pathogenic Leptospira spp. that infect the straw-colored fruit bat (Eidolon helvum). We captured this bat species, which is widely distributed in Africa, in Zambia during 2008–2013. We detected the flagellin B gene (flaB) from pathogenic Leptospira spp. in kidney samples from 79 of 529 E. helvum (14.9%) bats. Phylogenetic analysis of 70 flaB fragments amplified from E. helvum samples and previously reported sequences, revealed that 12 of the fragments grouped with Leptospira borgpetersenii and Leptospira kirschneri; however, the remaining 58 flaB fragments appeared not to be associated with any reported species. Additionally, the 16S ribosomal RNA gene (rrs) amplified from 27 randomly chosen flaB-positive samples was compared with previously reported sequences, including bat-derived Leptospira spp. All 27 rrs fragments clustered into a pathogenic group. Eight fragments were located in unique branches, the other 19 fragments were closely related to Leptospira spp. detected in bats. These results show that rrs sequences in bats are genetically related to each other without regional variation, suggesting that Leptospira are evolutionarily well-adapted to bats and have uniquely evolved in the bat population. Our study indicates that pathogenic Leptospira spp. in E. helvum in Zambia have unique genotypes.
Rights: https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/75551
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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