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Neutron crystallographic study of heterotrimeric glutamine amidotransferase CAB

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Title: Neutron crystallographic study of heterotrimeric glutamine amidotransferase CAB
Authors: Li, Long Browse this author
Adachi, Motoyasu Browse this author →KAKEN DB
Yu, Jian Browse this author
Kato, Koji Browse this author
Shinoda, Akira Browse this author
Ostermann, Andreas Browse this author
Schrader, Tobias E. Browse this author
Ose, Toyoyuki Browse this author →KAKEN DB
Yao, Min Browse this author →KAKEN DB
Keywords: glutamine amidotransferase CAB
ammonia-self-sufficient mechanism
ammonia channel
neutron diffraction
Issue Date: Mar-2019
Publisher: International Union of Crystallography
Journal Title: Acta crystallographica Section F
Volume: 75
Start Page: 193
End Page: 196
Publisher DOI: 10.1107/S2053230X19000220
Abstract: Heterotrimeric glutamine amidotransferase CAB (GatCAB) possesses an ammonia-self-sufficient mechanism in which ammonia is produced and used in the inner complex by GatA and GatB, respectively. The X-ray structure of GatCAB revealed that the two identified active sites of GatA and GatB are markedly distant, but are connected in the complex by a channel of 30 angstrom in length. In order to clarify whether ammonia is transferred through this channel in GatCAB by visualizing ammonia, neutron diffraction studies are indispensable. Here, GatCAB crystals were grown to approximate dimensions of 2.8 x 0.8 x 0.8 mm (a volume of 1.8 mm 3) with the aid of a polymer using microseeding and macroseeding processes. Monochromatic neutron diffraction data were collected using the neutron single-crystal diffractometer BIODIFF at the Heinz Maier-Leibnitz Zentrum, Germany. The GatCAB crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 74.6, b = 94.5, c = 182.5 angstrom and with one GatCAB complex (molecular mass 119 kDa) in the asymmetric unit. This study represented a challenge in current neutron diffraction technology.
Rights: © International Union of Crystallography
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 姚 閔

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