HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >

Requirement for Different Normalization Genes for Quantitative Gene Expression Analysis Under Abiotic Stress Conditions in ‘Bangia’ sp. ESS1

Creative Commons License

Files in This Item:

The file(s) associated with this item can be obtained from the following URL:https://norcaloa.com/ARMS/articles-in-press/ARMS-203037


Title: Requirement for Different Normalization Genes for Quantitative Gene Expression Analysis Under Abiotic Stress Conditions in ‘Bangia’ sp. ESS1
Authors: Li, Chengze Browse this author
Irie, Ryunosuke Browse this author
Shimada, Satoshi Browse this author
Mikami, Koji Browse this author →KAKEN DB
Keywords: Bangia
Gene Expression
Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-PCR)
Red Alga
Reference Gene
Stress Response
Issue Date: 28-Aug-2019
Publisher: Northern California Open Access Publications
Journal Title: Journal of Aquatic Research and Marine Sciences
Volume: 2019
Issue: 3
Start Page: 194
End Page: 205
Abstract: Quantitative gene expression analysis is indispensable for accurately characterizing the expression levels of genes involved in the response and acclimation of algae to environmental stresses. However, appropriate reference genes in the genus Bangia (order Bangiales) for quantification of stress-inducible gene expression are not well described. Thus, we evaluated the suitability of genes encoding 18S ribosomal RNA (18S rRNA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin, tubulin, elongation factor 1 (EF1), and 60S ribosomal RNA (60S rRNA) as reference genes for real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) under various stress conditions in ‘Bangia’ sp. ESS1, classified into the ‘Bangia’ 2 group. Our results showed that the 60S rRNA and GAPDH genes were most suitable for gene expression analyses in response to nutrient deficiency, high salinity, and temperature stress, whereas normalization of desiccation-inducible expression required use of the EF1 and tubulin genes. These findings indicate that reference genes for analysis of stress-inducible gene expression by qRT-PCR should be selected according to the environmental stress being studied. Our efforts to identify easily amplified reference genes for various stress responses provide novel insights into the selection of reference genes for seaweeds.
Rights: https://creativecommons.org/licenses/by/4.0/
Publisher URI: https://norcaloa.com/ARMS/articles-in-press/ARMS-203037
Type: article
URI: http://hdl.handle.net/2115/75617
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三上 浩司

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University