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A Simplified Gas Chromatographic Fatty-Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae

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Title: A Simplified Gas Chromatographic Fatty-Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae
Authors: Matsumoto, Yuko Browse this author
Ando, Yasuhiro Browse this author →KAKEN DB
Hiraoka, Yuko Browse this author
Tawa, Atsushi Browse this author
Ohshimo, Seiji Browse this author
Keywords: Fatty acid
Gas chromatography
Larval fish
Methyl ester
Methylation
Saponification
Issue Date: Sep-2018
Publisher: John Wiley & Sons
Journal Title: Lipids
Volume: 53
Issue: 9
Start Page: 919
End Page: 929
Publisher DOI: 10.1002/lipd.12098
Abstract: A method for the direct preparation of fatty‐acid methyl esters (FAME) was simplified for fatty‐acid analysis of a single fish larva using gas chromatography (GC). The method included the isolation of a larval trunk and drying in a glass vial, followed by saponification of all the contents without prior lipid extraction. Thereafter, the fatty acids released were methylated by trimethylsilyldiazomethane. This method has advantages over another method, direct acid‐catalyzed transesterification, because both the saponification and methylation at room temperature can reduce loss of unsaturated fatty acids and formation of artifacts unavoidable in acidic reaction at high temperature. GC of the products showed that the simplified method can yield methyl esters without artifacts interfering analysis. More than 50 fatty acids were determined, which are twice as many as those previously analyzed using high‐performance liquid chromatography. Observation of consistent small impurities in GC of blank tests allowed the accurate determination of fatty acids by correcting the peak areas. Dry matter weights (<3 mg) and the total fatty‐acid contents displayed a linear relationship. Fatty‐acid analysis of wild larvae of bluefin tuna, yellowfin tuna, and skipjack tuna collected from the waters around Japan (n = 100) revealed that the eicosapentaenoic acid (EPA) level in bluefin tuna collected from the Japan Sea was significantly higher than that in the three species collected from Nansei Islands. The simplified direct saponification/methylation method will be a powerful tool for investigating growth and survival of individual larval tuna and other fish species.
Rights: This is the peer reviewed version of the following article: Matsumoto, Y. , Ando, Y. , Hiraoka, Y. , Tawa, A. and Ohshimo, S. (2018), A Simplified Gas Chromatographic Fatty‐Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae. Lipids, 53: 919-929. doi:10.1002/lipd.12098, which has been published in final form at https://doi.org/10.1002/lipd.12098. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
URI: http://hdl.handle.net/2115/75787
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 安藤 靖浩

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