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MRL/MpJ mice produce more oocytes and exhibit impaired fertilisation and accelerated luteinisation after superovulation treatment

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Title: MRL/MpJ mice produce more oocytes and exhibit impaired fertilisation and accelerated luteinisation after superovulation treatment
Authors: Hosotani, Marina Browse this author
Ichii, Osamu Browse this author →KAKEN DB
Nakamura, Teppei Browse this author
Masum, Md Abdul Browse this author
Otani, Yuki Browse this author
Otsuka-Kanazawa, Saori Browse this author
Elewa, Yaser H. A. Browse this author
Kon, Yasuhiro Browse this author →KAKEN DB
Keywords: extracellular matrix
invitro fertilisation
Issue Date: Mar-2019
Publisher: CSIRO Publishing
Journal Title: Reproduction, Fertility and Development
Volume: 31
Issue: 4
Start Page: 760
End Page: 773
Publisher DOI: 10.1071/RD18319
Abstract: MRL/MpJ mice exhibit distinct phenotypes in several biological processes, including wound healing. Herein we report two unique phenotypes in the female reproductive system of MRL/MpJ mice that affect ovulation and luteinisation. We found that superovulation treatment resulted in the production of significantly more oocytes in MRL/MpJ than C57BL/6 mice (71.0 +/- 13.4 vs 26.8 +/- 2.8 respectively). However, no exon mutations were detected in genes coding for female reproductive hormones or their receptors in MRL/MpJ mice. In addition, the fertilisation rate was lower for ovulated oocytes from MRL/MpJ than C57BL/6 mice, with most of the fertilised oocytes showing abnormal morphology, characterised by deformation and cytolysis. Histological tracing of luteinisation showed that MRL/MpJ mice formed corpora lutea within 36h after ovulation, whereas C57BL/6 mice were still at the corpora haemorrhagica formation stage after 36h. The balance between the expression of matrix metalloproteinases and their tissue inhibitors shifted towards the former earlier after ovulation in MRL/MpJ than C57BL/6 mice. This result indicates a possible link between accelerated extracellular matrix remodelling in the ovulated or ruptured follicles and luteinisation in MRL/MpJ mice. Together, these findings reveal novel phenotypes in MRL/MpJ mice that provide novel insights into reproductive biology.
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 昆 泰寛

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