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Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/75973

Title: Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells
Authors: Takagi, Mikio Browse this author
Ikegawa, Masaya Browse this author
Shimada, Takashi Browse this author
Ishikawa, Susumu Browse this author
Kajita, Mihoko Browse this author →KAKEN DB
Maruyama, Takeshi Browse this author
Kamasaki, Tomoko Browse this author
Fujita, Yasuyuki Browse this author →KAKEN DB
Issue Date: Nov-2018
Publisher: John Wiley & Sons
Journal Title: Genes to cells
Volume: 23
Issue: 11
Start Page: 974
End Page: 981
Publisher DOI: 10.1111/gtc.12643
Abstract: At the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cells are often apically eliminated from epithelial tissues. However, the underlying molecular mechanisms of this cancer preventive phenomenon still remain elusive. In this study, we first demonstrate that myosin-II accumulates in Src-transformed cells when they are surrounded by normal epithelial cells. Knock-down of the heavy chains of myosin-II substantially diminishes apical extrusion of Src cells, suggesting that accumulated myosin-II positively regulates the apical elimination of transformed cells. Furthermore, we have identified β-spectrin as a myosin-II-binding protein under the coculture of normal and Src-transformed epithelial cells. β-spectrin is also accumulated in Src cells that are surrounded by normal cells, and the β-spectrin accumulation is regulated by myosin-II. Moreover, knock-down of β-spectrin significantly suppresses apical extrusion of Src cells. Collectively, these results indicate that accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. Further elucidation of the molecular mechanisms of apical extrusion would lead to the establishment of a novel type of cancer preventive medicine.
Rights: This is the peer reviewed version of the following article: Takagi M, Ikegawa M, Shimada T, et al., Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells, Genes Cells, 2018;23:974–981, which has been published in final form at https://doi.org/10.1111/gtc.12643. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
URI: http://hdl.handle.net/2115/75973
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藤田 恭之

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