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Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry

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Title: Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry
Authors: Chen, Zhen Browse this author
Wu, Yue Browse this author
Shrestha, Rojeet Browse this author
Gao, Zijun Browse this author
Zhao, Yaoyao Browse this author
Miura, Yusuke Browse this author
Tamakoshi, Akiko Browse this author →KAKEN DB
Chiba, Hitoshi Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Keywords: Short-chain fatty acid
butanoic acid (FA 4:0)
caproic acid (FA 6:0)
Issue Date: Mar-2019
Publisher: Sage
Journal Title: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
Volume: 56
Issue: 2
Start Page: 190
End Page: 197
Publisher DOI: 10.1177/0004563218801393
PMID: 30185055
Abstract: BACKGROUND:Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited. METHODS:Blood samples from human subjects ( n = 547, male/female = 246/301, age 58.85 ± 12.57) were collected. Saponification was applied to obtain total fatty acid. After derivatization by 2-nitrophenylhydrazine, fatty acid 4:0 and fatty acid 6:0 were measured by liquid chromatography-mass spectrometry. RESULTS:The developed method exhibited good linearity (R2 = 0.9996 for both). All the coefficients of variation of reproducibility and accuracy for fatty acid 4:0 and fatty acid 6:0 ranged 3.0%-6.1%, with the average recoveries of 87.8%-102.4% and 92.2%-98.2%, respectively. In all the samples, the concentration of fatty acid 4:0 (162.4 ± 76.4 μmol/L) was significantly higher than fatty acid 6:0 (2.0 ± 2.5 μmol/L, P < 0.001). Furthermore, the esterified form was predominant in both fatty acid 4:0 and fatty acid 6:0 (98.2% and 82.4% of total fatty acids, respectively). Besides, short-chain fatty acids showed no significant differences with regard to sex or age differences. CONCLUSION:This developed liquid chromatography-mass spectrometry method is convenient and reliable, which might be useful for monitoring the variations of short-chain fatty acids in blood.
Rights: Chen Zhen, Yue Wu, Rojeet Shrestha, Zijun Gao, Yaoyao Zhao, Yusuke Miura, Akiko Tamakoshi, Hitoshi Chiba, and Shu-Ping Hui, Determination of Total, Free and Esterified Short-Chain Fatty Acid in Human Serum by Liquid Chromatography-Mass Spectrometry, Annals of Clinical Biochemistry (v.56, no. 2): pp. 190–97. Copyright © 2019 (SAGE). Reprinted by permission of SAGE Publications. DOI: 10.1177/0004563218801393.
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 陳 震

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