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Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry
| Title: | Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry |
| Authors: | Chen, Zhen Browse this author | | Wu, Yue Browse this author | | Shrestha, Rojeet Browse this author | | Gao, Zijun Browse this author | | Zhao, Yaoyao Browse this author | | Miura, Yusuke Browse this author | | Tamakoshi, Akiko Browse this author →KAKEN DB | | Chiba, Hitoshi Browse this author →KAKEN DB | | Hui, Shu-Ping Browse this author →KAKEN DB |
| Keywords: | Short-chain fatty acid | | butanoic acid (FA 4:0) | | caproic acid (FA 6:0) | | LC-MS/MS | | serum |
| Issue Date: | Mar-2019 |
| Publisher: | Sage |
| Journal Title: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine |
| Volume: | 56 |
| Issue: | 2 |
| Start Page: | 190 |
| End Page: | 197 |
| Publisher DOI: | 10.1177/0004563218801393 |
| PMID: | 30185055 |
| Abstract: | BACKGROUND:Short-chain fatty acids are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevent type 2 diabetes and some cancers. However, reports on the determination of these short-chain fatty acids in human serum are limited. METHODS:Blood samples from human subjects ( n = 547, male/female = 246/301, age 58.85 ± 12.57) were collected. Saponification was applied to obtain total fatty acid. After derivatization by 2-nitrophenylhydrazine, fatty acid 4:0 and fatty acid 6:0 were measured by liquid chromatography-mass spectrometry. RESULTS:The developed method exhibited good linearity (R2 = 0.9996 for both). All the coefficients of variation of reproducibility and accuracy for fatty acid 4:0 and fatty acid 6:0 ranged 3.0%-6.1%, with the average recoveries of 87.8%-102.4% and 92.2%-98.2%, respectively. In all the samples, the concentration of fatty acid 4:0 (162.4 ± 76.4 μmol/L) was significantly higher than fatty acid 6:0 (2.0 ± 2.5 μmol/L, P < 0.001). Furthermore, the esterified form was predominant in both fatty acid 4:0 and fatty acid 6:0 (98.2% and 82.4% of total fatty acids, respectively). Besides, short-chain fatty acids showed no significant differences with regard to sex or age differences. CONCLUSION:This developed liquid chromatography-mass spectrometry method is convenient and reliable, which might be useful for monitoring the variations of short-chain fatty acids in blood. |
| Rights: | Chen Zhen, Yue Wu, Rojeet Shrestha, Zijun Gao, Yaoyao Zhao, Yusuke Miura, Akiko Tamakoshi, Hitoshi Chiba, and Shu-Ping Hui, Determination of Total, Free and Esterified Short-Chain Fatty Acid in Human Serum by Liquid Chromatography-Mass Spectrometry, Annals of Clinical Biochemistry (v.56, no. 2): pp. 190–97. Copyright © 2019 (SAGE). Reprinted by permission of SAGE Publications. DOI: 10.1177/0004563218801393. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/76142 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 陳 震
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