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Insight into genetic diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka reveals predominance of the Euro-American lineage

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Title: Insight into genetic diversity of Mycobacterium tuberculosis in Kandy, Sri Lanka reveals predominance of the Euro-American lineage
Authors: Mendis, Charitha Browse this author
Thevanesam, Vasanthi Browse this author
Kumara, Athula Browse this author
Wickramasinghe, Susiji Browse this author
Madegedara, Dushantha Browse this author
Gamage, Chandika Browse this author
Gordon, Stephen V. Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Ratnatunga, Champa Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Keywords: Mycobacterium tuberculosis
Lineage 4 (Euro-American lineage)
Sri Lanka
Issue Date: Oct-2019
Publisher: Elsevier
Journal Title: International journal of infectious diseases
Volume: 87
Start Page: 84
End Page: 91
Publisher DOI: 10.1016/j.ijid.2019.07.001
PMID: 31299365
Abstract: Objective: Sri Lanka is a country where the molecular epidemiology of Mycobacterium tuberculosis (MTB) is poorly explored. Therefore, this study was performed to identify circulating lineages/sub-lineages of MTB and their transmission patterns. Methods: DNA was extracted from 89 isolates of MTB collected during 2012 and 2013 from new pulmonary tuberculosis patients in Kandy, Sri Lanka and analyzed by spoligotyping, large sequence polymorphism (LSP), mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing, and drug resistance-associated gene sequencing. Results: The predominant lineage was lineage 4 (Euro-American, 45.9%), followed by lineage 1 (Indo-Oceanic, 29.4%), lineage 2 (East-Asian, 23.5%), and lineage 3 (Central-Asian, 1.2%). Among 26 spoligotype patterns, eight were undesignated or new types and seven of these belonged to lineage 4. Undesignated lineage 4/SIT124 (n = 2/8) and SIT3234 (n = 8/8) clustered together based on 24-locus MIRU-VNTR typing. The dominant sub-lineage was Beijing/SIT1 (n = 19), with the isoniazid resistance katG G944C mutation (Ser315Thr) detected in two of them. Conclusions: The population structure of MTB in Kandy, Sri Lanka was different from that in the South Asian region. The clonal expansion of locally evolved lineage 4/SIT3234 and detection of the premultidrug resistant Beijing isolates from new tuberculosis patients is alarming and will require continuous monitoring. (C) 2019 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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