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Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae

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Title: Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae
Authors: Matsushita, Mizue Browse this author
Okubo, Torahiko Browse this author
Hasegawa, Takaki Browse this author
Matsuo, Junji Browse this author →KAKEN DB
Watanabe, Takanori Browse this author
Iwasaki, Sumio Browse this author
Fukumoto, Tatsuya Browse this author
Hayasaka, Kasumi Browse this author
Akizawa, Kozi Browse this author
Shimizu, Chikara Browse this author →KAKEN DB
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Keywords: Aeromonas caviae
ciliates
Escherichia coli
gene transfer
Issue Date: Nov-2018
Publisher: John Wiley & Sons
Journal Title: Microbiology and immunology
Volume: 62
Issue: 11
Start Page: 720
End Page: 728
Publisher DOI: 10.1111/1348-0421.12656
PMID: 30357893
Abstract: Tetrahymena can facilitate plasmid transfer among Escherichia coli or from E. coli to Salmonella Enteritidis via vesicle accumulation. In this study, whether ciliates promote the interactive transfer of plasmids encoding bla(IMP-1) between fecal E. coli and environmental Aeromonas caviae was investigated. Both bacteria were mixed with or without ciliates and incubated overnight at 30 degrees C. The frequency of plasmid-acquired bacteria was estimated by colony counts using an agar plate containing ceftazidim (CAZ) followed by determination of the minimum inhibitory concentration (MIC). Cultures containing ciliates interactively transferred the plasmid between E. coli and Aeromonas with a frequency of 10(-4) to 10(-5). All plasmid-acquired bacteria showed a MIC against CAZ of >128 mu g/mL and the plasmid transfer was confirmed by PCR amplification of the bla(IMP-1) gene. Fluorescent observation showed that both bacteria accumulated in the same vesicle and that transwell sequestering significantly decreased the transfer frequency. Although ciliates preferentially ingested E. coli rather than A. caviae, both bacteria were co-localized into the same vesicles of ciliates, indicating that their meeting is associated with the gene transfer. Thus, ciliates interactively promote plasmid transfer between E. coli and A. caviae. The results of this study will facilitate control of the spread of multiple-antibiotic resistant bacteria.
Rights: This is the peer reviewed version of the following article: Matsushita, M., Okubo, T., Hasegawa, T., Matsuo, J., Watanabe, T., Iwasaki, S., Fukumoto, T., Hayasaka, K., Akizawa, K., Shimizu, C., and Yamaguchi, H. (2018), Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae., Microbiology and Immunology, 62(11): 720–728., which has been published in final form at https://doi.org/10.1111/1348-0421.12656. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/76240
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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