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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae
| Title: | Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae |
| Authors: | Matsushita, Mizue Browse this author | | Okubo, Torahiko Browse this author | | Hasegawa, Takaki Browse this author | | Matsuo, Junji Browse this author →KAKEN DB | | Watanabe, Takanori Browse this author | | Iwasaki, Sumio Browse this author | | Fukumoto, Tatsuya Browse this author | | Hayasaka, Kasumi Browse this author | | Akizawa, Kozi Browse this author | | Shimizu, Chikara Browse this author →KAKEN DB | | Yamaguchi, Hiroyuki Browse this author →KAKEN DB |
| Keywords: | Aeromonas caviae | | ciliates | | Escherichia coli | | gene transfer |
| Issue Date: | Nov-2018 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Microbiology and immunology |
| Volume: | 62 |
| Issue: | 11 |
| Start Page: | 720 |
| End Page: | 728 |
| Publisher DOI: | 10.1111/1348-0421.12656 |
| PMID: | 30357893 |
| Abstract: | Tetrahymena can facilitate plasmid transfer among Escherichia coli or from E. coli to Salmonella Enteritidis via vesicle accumulation. In this study, whether ciliates promote the interactive transfer of plasmids encoding bla(IMP-1) between fecal E. coli and environmental Aeromonas caviae was investigated. Both bacteria were mixed with or without ciliates and incubated overnight at 30 degrees C. The frequency of plasmid-acquired bacteria was estimated by colony counts using an agar plate containing ceftazidim (CAZ) followed by determination of the minimum inhibitory concentration (MIC). Cultures containing ciliates interactively transferred the plasmid between E. coli and Aeromonas with a frequency of 10(-4) to 10(-5). All plasmid-acquired bacteria showed a MIC against CAZ of >128 mu g/mL and the plasmid transfer was confirmed by PCR amplification of the bla(IMP-1) gene. Fluorescent observation showed that both bacteria accumulated in the same vesicle and that transwell sequestering significantly decreased the transfer frequency. Although ciliates preferentially ingested E. coli rather than A. caviae, both bacteria were co-localized into the same vesicles of ciliates, indicating that their meeting is associated with the gene transfer. Thus, ciliates interactively promote plasmid transfer between E. coli and A. caviae. The results of this study will facilitate control of the spread of multiple-antibiotic resistant bacteria. |
| Rights: | This is the peer reviewed version of the following article: Matsushita, M., Okubo, T., Hasegawa, T., Matsuo, J., Watanabe, T., Iwasaki, S., Fukumoto, T., Hayasaka, K., Akizawa, K., Shimizu, C., and Yamaguchi, H. (2018), Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae., Microbiology and Immunology, 62(11): 720–728., which has been published in final form at https://doi.org/10.1111/1348-0421.12656. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/76240 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山口 博之
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