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Hokkaido University Collection of Scholarly and Academic Papers >
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Simultaneous collection of the portal and superior vena cava blood in conscious rats defined that intestinal epithelium is the major site of glucuronidation, but not sulfation and methylation, of quercetin
| Title: | Simultaneous collection of the portal and superior vena cava blood in conscious rats defined that intestinal epithelium is the major site of glucuronidation, but not sulfation and methylation, of quercetin |
| Authors: | Tanaka, Seiya Browse this author | | Oyama, Manami Browse this author | | Nishikawa, Miyu Browse this author →KAKEN DB | | Ikushiro, Shinichi Browse this author | | Hara, Hiroshi Browse this author →KAKEN DB |
| Keywords: | Quercetin | | glucuronide | | sulfate | | conjugation | | porto-venous difference |
| Issue Date: | 2-Dec-2018 |
| Publisher: | Taylor & Francis |
| Journal Title: | Bioscience biotechnology and biochemistry |
| Volume: | 82 |
| Issue: | 12 |
| Start Page: | 2118 |
| End Page: | 2129 |
| Publisher DOI: | 10.1080/09168451.2018.1515615 |
| PMID: | 30200823 |
| Abstract: | Quercetin is a flavonoid with many physiological effects. Absorbed quercetin is rapidly conjugated in the intestinal epithelium and liver. Different positional isomers of quercetin conjugates have different physiological properties. However, the mechanisms of quercetin conjugation in the intestine are not fully clarified. We examined the regioselective quercetin conjugate formation in the intestine after oral administration of quercetin glycosides, by simultaneous sampling of blood from the portal vein and superior vena cava, and quantifying various positional isomers of quercetin glucuronides and sulfates in conscious rats. Concentrations of quercetin glucuronides were higher in blood from the portal vein than the superior vena cava, showing that glucuronidation mainly occurred in the intestine. Such differences were not observed for quercetin sulfates. Regioselectivity of the intestinal glucuronidation in quercetin hydroxyl groups were 7->3'->3->4'-OH. Quercetin was mainly sulfated on 3'-OH at 30 min, but on 4'-OH at 240 min. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience Biotechnology and Biochemistry on 10 Sep 2018, available online: shttps://doi.org/10.1080/09168451.2018.1515615 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/76243 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 原 博
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