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Hokkaido University Collection of Scholarly and Academic Papers >
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Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)
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| Title: | Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus) |
| Authors: | Paudel, Sarad Browse this author →KAKEN DB | | Villanueva, A. Marvin Browse this author | | Mikota, K. Susan Browse this author | | Nakajima, Chie Browse this author →KAKEN DB | | Gairhe, P. Kamal Browse this author | | Subedi, Suraj Browse this author | | Rayamajhi, Nabin Browse this author | | Sashika, Mariko Browse this author | | Shimozuru, Michito Browse this author →KAKEN DB | | Matsuba, Takashi Browse this author →KAKEN DB | | Suzuki, Yasuhiko Browse this author →KAKEN DB | | Tsubota, Toshio Browse this author →KAKEN DB |
| Keywords: | Asian elephant | | interferon-γ | | mitogen | | sandwich ELISA |
| Issue Date: | Jul-2016 |
| Publisher: | The Japanese Society of Veterinary Science |
| Journal Title: | Journal of Veterinary Medical Science |
| Volume: | 78 |
| Issue: | 7 |
| Start Page: | 1117 |
| End Page: | 1121 |
| Publisher DOI: | 10.1292/jvms.15-0701 |
| PMID: | 26983683 |
| Abstract: | We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants. |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/76354 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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