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Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)

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Title: Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)
Authors: Paudel, Sarad Browse this author →KAKEN DB
Villanueva, A. Marvin Browse this author
Mikota, K. Susan Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Gairhe, P. Kamal Browse this author
Subedi, Suraj Browse this author
Rayamajhi, Nabin Browse this author
Sashika, Mariko Browse this author
Shimozuru, Michito Browse this author →KAKEN DB
Matsuba, Takashi Browse this author →KAKEN DB
Suzuki, Yasuhiko Browse this author →KAKEN DB
Tsubota, Toshio Browse this author →KAKEN DB
Keywords: Asian elephant
interferon-γ
mitogen
sandwich ELISA
Issue Date: Jul-2016
Publisher: The Japanese Society of Veterinary Science
Journal Title: Journal of Veterinary Medical Science
Volume: 78
Issue: 7
Start Page: 1117
End Page: 1121
Publisher DOI: 10.1292/jvms.15-0701
PMID: 26983683
Abstract: We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants.
Rights: https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/76354
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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