| Title: | Host-derived apolipoproteins play comparable roles with viral secretory proteins Erns and NS1 in the infectious particle formation of Flaviviridae |
| Authors: | Fukuhara, Takasuke Browse this author →KAKEN DB |
| Tamura, Tomokazu Browse this author |
| Ono, Chikako Browse this author →KAKEN DB |
| Shiokawa, Mai Browse this author |
| Mori, Hiroyuki Browse this author |
| Uemura, Kentaro Browse this author |
| Yamamoto, Satomi Browse this author |
| Kurihara, Takeshi Browse this author |
| Okamoto, Toru Browse this author →KAKEN DB |
| Suzuki, Ryosuke Browse this author |
| Yoshii, Kentaro Browse this author →KAKEN DB |
| Kurosu, Takeshi Browse this author →KAKEN DB |
| Igarashi, Manabu Browse this author →KAKEN DB |
| Aoki, Hiroshi Browse this author →KAKEN DB |
| Sakoda, Yoshihiro Browse this author →KAKEN DB |
| Matsuura, Yoshiharu Browse this author →KAKEN DB |
| Issue Date: | 23-Jun-2017 |
| Publisher: | PLOS |
| Journal Title: | PLoS pathogens |
| Volume: | 13 |
| Issue: | 6 |
| Start Page: | e1006475 |
| Publisher DOI: | 10.1371/journal.ppat.1006475 |
| PMID: | 28644867 |
| Abstract: | Amphipathic α-helices of exchangeable apolipoproteins have shown to play crucial roles in the formation of infectious hepatitis C virus (HCV) particles through the interaction with viral particles. Among the Flaviviridae members, pestivirus and flavivirus possess a viral structural protein Erns or a non-structural protein 1 (NS1) as secretory glycoproteins, respectively, while Hepacivirus including HCV has no secretory glycoprotein. In case of pestivirus replication, the C-terminal long amphipathic α-helices of Erns are important for anchoring to viral membrane. Here we show that host-derived apolipoproteins play functional roles similar to those of virally encoded Erns and NS1 in the formation of infectious particles. We examined whether Erns and NS1 could compensate for the role of apolipoproteins in particle formation of HCV in apolipoprotein B (ApoB) and ApoE double-knockout Huh7 (BE-KO), and non-hepatic 293T cells. We found that exogenous expression of either Erns or NS1 rescued infectious particle formation of HCV in the BE-KO and 293T cells. In addition, expression of apolipoproteins or NS1 partially rescued the production of infectious pestivirus particles in cells upon electroporation with an Erns-deleted non-infectious RNA. As with exchangeable apolipoproteins, the C-terminal amphipathic α-helices of Erns play the functional roles in the formation of infectious HCV or pestivirus particles. These results strongly suggest that the host- and virus-derived secretory glycoproteins have overlapping roles in the viral life cycle of Flaviviridae, especially in the maturation of infectious particles, while Erns and NS1 also participate in replication complex formation and viral entry, respectively. Considering the abundant hepatic expression and liver-specific propagation of these apolipoproteins, HCV might have evolved to utilize them in the formation of infectious particles through deletion of a secretory viral glycoprotein gene. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/76370 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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