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Significance of CCN2 expression in bovine preimplantation development

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Title: Significance of CCN2 expression in bovine preimplantation development
Authors: Akizawa, Hiroki Browse this author
Yanagawa, Yojiro Browse this author
Nagano, Masashi Browse this author →KAKEN DB
Bai, Hanako Browse this author →KAKEN DB
Takahashi, Masashi Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: blastocyst
cell-lineage specification
pluripotency-related genes
Issue Date: Jan-2019
Publisher: John Wiley & Sons
Journal Title: Animal science journal
Volume: 90
Issue: 1
Start Page: 49
End Page: 54
Publisher DOI: 10.1111/asj.13126
PMID: 30358017
Abstract: In mammalian preimplantation development, the first cell lineage segregation occurs during the blastocyst stage, when the inner cell mass and trophectoderm (TE) differentiate. Species-specific analyses are essential to elucidate the molecular mechanisms that underlie this process, since they differ between various species. We previously showed that the reciprocal regulation of CCN2 and TEAD4 is required for proper TE differentiation in bovine blastocysts; however, the function of CCN2 during early embryogenesis has remained otherwise elusive. The present study assessed the spatiotemporal expression dynamics of CCN2 in bovine embryos, and evaluated how changes to CCN2 expression (using a CCN2 knockdown (KD) blastocyst model) regulate the expression of pluripotency-related genes such as OCT4 and NANOG. The conducted quantitative PCR analysis revealed that CCN2 mRNA was expressed in bovine oocytes (at the metaphase stage of their second meiosis) and embryos. Similarly, immunostaining detected both cytoplasmic and nuclear CCN2 at all analyzed oocyte and embryonic stages. Finally, both OCT4 and NANOG expression levels were shown to be significantly reduced in CCN2 KD blastocysts. Together, these results demonstrate that bovine CCN2 exhibits unique expression patterns during preimplantation development, and is required for the proper expression of key regulatory genes in bovine blastocysts.
Rights: This is the peer reviewed version of the following article: Hiroki Akizawa, Yojiro Yanagawa, Masashi Nagano, Hanako Bai, Masashi Takahashi, Manabu Kawahara (2019). Significance of CCN2 expression in bovine preimplantation development. Animal science journal, 90(1), 49-54, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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