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Identification and characterization of a semi-dominant restorer-of-fertility 1 allele in sugar beet (Beta vulgaris)

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Title: Identification and characterization of a semi-dominant restorer-of-fertility 1 allele in sugar beet (Beta vulgaris)
Authors: Arakawa, Takumi Browse this author
Ue, Sachiyo Browse this author
Sano, Chihiro Browse this author
Matsunaga, Muneyuki Browse this author
Kagami, Hiroyo Browse this author
Yoshida, Yu Browse this author
Kuroda, Yosuke Browse this author
Taguchi, Kazunori Browse this author
Kitazaki, Kazuyoshi Browse this author
Kubo, Tomohiko Browse this author →KAKEN DB
Keywords: nuclear-cytoplasmic interaction
restorer of fertility
gene dose
cytoplasmic male sterility
anther development
Issue Date: Jan-2019
Publisher: Springer Berlin Heidelberg
Journal Title: Theoretical and Applied Genetics
Volume: 132
Issue: 1
Start Page: 227
End Page: 240
Publisher DOI: 10.1007/s00122-018-3211-6
PMID: 30341492
Abstract: Key message: The sugar beet Rf1 locus has a number of molecular variants. We found that one of the molecular variants is a weak allele of a previously identified allele. Abstract: Male sterility (MS) caused by nuclear-mitochondrial interaction is called cytoplasmic male sterility (CMS) in which MS-inducing mitochondria are suppressed by a nuclear gene, restorer-of-fertility. Rf and rf are the suppressing and non-suppressing alleles, respectively. This dichotomic view, however, seems somewhat unsatisfactory to explain the recently discovered molecular diversity of Rf loci. In the present study, we first identified sugar beet line NK-305 as a new source of Rf1. Our crossing experiment revealed that NK-305 Rf1 is likely a semi-dominant allele that restores partial fertility when heterozygous but full fertility when homozygous, whereas Rf1 from another sugar beet line appeared to be a dominant allele. Proper degeneration of anther tapetum is a prerequisite for pollen development; thus, we compared tapetal degeneration in the NK-305 Rf1 heterozygote and the homozygote. Degeneration occurred in both genotypes but to a lesser extent in the heterozygote, suggesting an association between NK-305 Rf1 dose and incompleteness of tapetal degeneration leading to partial fertility. Our protein analyses revealed a quantitative correlation between NK-305 Rf1 dose and a reduction in the accumulation of a 250 kDa mitochondrial protein complex consisting of a CMS-specific mitochondrial protein encoded by MS-inducing mitochondria. The abundance of Rf1 transcripts correlated with NK-305 Rf1 dose. The molecular organization of NK-305 Rf1 suggested that this allele evolved through intergenic recombination. We propose that the sugar beet Rf1 locus has a series of multiple alleles that differ in their ability to restore fertility and are reflective of the complexity of Rf evolution. © Springer-Verlag GmbH Germany, part of Springer Nature 2018
Rights: The final publication is available at, This is a post-peer-review, pre-copyedit version of an article published in Theoretical and Applied Genetics. The final authenticated version is available online at:
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 久保 友彦

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