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Rapid identification of genes controlling virulence and immunity in malaria parasites

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Title: Rapid identification of genes controlling virulence and immunity in malaria parasites
Authors: Abkallo, Hussein M Browse this author
Martinelli, Axel Browse this author
Inoue, Megumi Browse this author
Ramaprasad, Abhinay Browse this author
Xangsayarath, Phonepadith Browse this author
Gitaka, Jesse Browse this author
Tang, Jianxia Browse this author
Yahata, Kazuhide Browse this author →KAKEN DB
Zoungrana, Augustin Browse this author
Mitaka, Hayato Browse this author
Acharjee, Arita Browse this author
Datta, Partha P Browse this author
Hunt, Paul Browse this author
Carter, Richard Browse this author
Kaneko, Osamu Browse this author →KAKEN DB
Mustonen, Ville Browse this author
Illingworth, Christopher J R Browse this author
Pain, Arnab Browse this author
Culleton, Richard Browse this author →KAKEN DB
Issue Date: 12-Jul-2017
Publisher: PLOS
Journal Title: PLoS Pathog
Volume: 13
Issue: 7
Start Page: e1006447
Publisher DOI: 10.1371/journal.ppat.1006447
PMID: 28704525
Abstract: Identifying the genetic determinants of phenotypes that impact disease severity is of fundamental importance for the design of new interventions against malaria. Here we present a rapid genome-wide approach capable of identifying multiple genetic drivers of medically relevant phenotypes within malaria parasites via a single experiment at single gene or allele resolution. In a proof of principle study, we found that a previously undescribed single nucleotide polymorphism in the binding domain of the erythrocyte binding like protein (EBL) conferred a dramatic change in red blood cell invasion in mutant rodent malaria parasites Plasmodium yoelii. In the same experiment, we implicated merozoite surface protein 1 (MSP1) and other polymorphic proteins, as the major targets of strain-specific immunity. Using allelic replacement, we provide functional validation of the substitution in the EBL gene controlling the growth rate in the blood stages of the parasites.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/76485
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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