| Title: | Rapid identification of genes controlling virulence and immunity in malaria parasites |
| Authors: | Abkallo, Hussein M Browse this author |
| Martinelli, Axel Browse this author |
| Inoue, Megumi Browse this author |
| Ramaprasad, Abhinay Browse this author |
| Xangsayarath, Phonepadith Browse this author |
| Gitaka, Jesse Browse this author |
| Tang, Jianxia Browse this author |
| Yahata, Kazuhide Browse this author →KAKEN DB |
| Zoungrana, Augustin Browse this author |
| Mitaka, Hayato Browse this author |
| Acharjee, Arita Browse this author |
| Datta, Partha P Browse this author |
| Hunt, Paul Browse this author |
| Carter, Richard Browse this author |
| Kaneko, Osamu Browse this author →KAKEN DB |
| Mustonen, Ville Browse this author |
| Illingworth, Christopher J R Browse this author |
| Pain, Arnab Browse this author |
| Culleton, Richard Browse this author →KAKEN DB |
| Issue Date: | 12-Jul-2017 |
| Publisher: | PLOS |
| Journal Title: | PLoS Pathog |
| Volume: | 13 |
| Issue: | 7 |
| Start Page: | e1006447 |
| Publisher DOI: | 10.1371/journal.ppat.1006447 |
| PMID: | 28704525 |
| Abstract: | Identifying the genetic determinants of phenotypes that impact disease severity is of fundamental importance for the design of new interventions against malaria. Here we present a rapid genome-wide approach capable of identifying multiple genetic drivers of medically relevant phenotypes within malaria parasites via a single experiment at single gene or allele resolution. In a proof of principle study, we found that a previously undescribed single nucleotide polymorphism in the binding domain of the erythrocyte binding like protein (EBL) conferred a dramatic change in red blood cell invasion in mutant rodent malaria parasites Plasmodium yoelii. In the same experiment, we implicated merozoite surface protein 1 (MSP1) and other polymorphic proteins, as the major targets of strain-specific immunity. Using allelic replacement, we provide functional validation of the substitution in the EBL gene controlling the growth rate in the blood stages of the parasites. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/76485 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
