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Characterization and expression profiles of small heat shock proteins in the marine red alga Pyropia yezoensis

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Title: Characterization and expression profiles of small heat shock proteins in the marine red alga Pyropia yezoensis
Authors: Uji, Toshiki Browse this author →KAKEN DB
Gondaira, Yohei Browse this author
Fukuda, Satoru Browse this author →KAKEN DB
Mizuta, Hiroyuki Browse this author →KAKEN DB
Saga, Naotsune Browse this author →KAKEN DB
Keywords: Pyropia yezoensis
Small heat shock proteins
Red algae
Abiotic stress
Plant growth hormone
Issue Date: Jan-2019
Publisher: Springer
Journal Title: Cell Stress and Chaperones
Volume: 24
Issue: 1
Start Page: 223
End Page: 233
Publisher DOI: 10.1007/s12192-018-00959-9
Abstract: Small heat shock proteins (sHSPs) are found in all three domains of life (Bacteria, Archaea, and Eukarya) and play a critical role in protecting organisms from a range of environmental stresses. However, little is known about their physiological functions in red algae. Therefore, we characterized the sHSPs (PysHSPs) in the red macroalga Pyropia yezoensis, which inhabits the upper intertidal zone where it experiences fluctuating stressful environmental conditions on a daily and seasonal basis, and examined their expression profiles at different developmental stages and under varying environmental conditions. We identified five PysHSPs (PysHSP18.8, 19.1, 19.2, 19.5, and 25.8). Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that expression of the genes PysHSP18.8, PysHSP19.5, and PysHSP25.8 was repressed at all the developmental stages under normal conditions, whereas PysHSP19.1 and PysHSP19.2 were overexpressed in mature gametophytes and sporophytes. Exposure of the gametophytes to high temperature, oxidative stress, or copper significantly increased the mRNA transcript levels of all the five genes, while exogenous application of the ethylene precursor 1-aminocylopropane-1-carboxylic acid (ACC) significantly increased the expression levels of PysHSP19.2, PysHSP19.5, and PysHSP25.8. These findings will help to further our understanding of the role of PysHSP genes and provide clues about how Pyropia species can adapt to the stressful conditions encountered in the upper intertidal zone during their life cycle.
Rights: This is a post-peer-review, pre-copyedit version of an article published in Cell Stress and Chaperones. The final authenticated version is available online at:
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宇治 利樹

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