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Flow cytometric identification and cell-line establishment of macrophages in naked mole-rats

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Title: Flow cytometric identification and cell-line establishment of macrophages in naked mole-rats
Authors: Wada, Haruka Browse this author →KAKEN DB
Shibata, Yuhei Browse this author
Abe, Yurika Browse this author
Otsuka, Ryo Browse this author
Eguchi, Nanami Browse this author
Kawamura, Yoshimi Browse this author →KAKEN DB
Oka, Kaori Browse this author
Baghdadi, Muhammad Browse this author →KAKEN DB
Atsumi, Tatsuya Browse this author →KAKEN DB
Miura, Kyoko Browse this author
Seino, Ken-ichiro Browse this author →KAKEN DB
Issue Date: 29-Nov-2019
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 9
Start Page: 17981
Publisher DOI: 10.1038/s41598-019-54442-1
Abstract: Naked mole rats (NMRs) have extraordinarily long lifespans and anti-tumorigenic capability. Recent studies of humans and mice have shown that many age-related diseases, including cancer, are strongly correlated with immunity, and macrophages play particularly important roles in immune regulation. Therefore, NMR macrophages may contribute to their unique phenotypes. However, studies of the roles of macrophages are limited by material restrictions and the lack of an established experimental strategy. In this study, we developed a flow cytometric strategy to identify NMR macrophages. The NMR macrophages were extractable using an off-the-shelf anti-CD11b antibody, M1/70, and forward/side scatter data obtained by flow cytometry. NMR macrophages proliferated in response to human/mouse recombinant M-CSF and engulfed Escherichia coli particles. Interestingly, the majority of NMR macrophages exhibited co-staining with an anti-NK1.1 antibody, PK136. NK1.1 antigen crosslinking with PK136 results in mouse NK cell stimulation; similarly, NMR macrophages proliferated in response to NK1.1 antibody treatment. Furthermore, we successfully established an NMR macrophage cell line, NPM1, by transduction of Simian virus 40 early region that proliferated indefinitely without cytokines and retained its phagocytotic capacity. The NPM1 would contribute to further studies on the immunity of NMRs.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/76504
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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