|
Hokkaido University Collection of Scholarly and Academic Papers >
Institute for Genetic Medicine >
Peer-reviewed Journal Articles, etc >
Flow cytometric identification and cell-line establishment of macrophages in naked mole-rats
This item is licensed under:Creative Commons Attribution 4.0 International
Title: | Flow cytometric identification and cell-line establishment of macrophages in naked mole-rats |
Authors: | Wada, Haruka Browse this author →KAKEN DB | Shibata, Yuhei Browse this author | Abe, Yurika Browse this author | Otsuka, Ryo Browse this author | Eguchi, Nanami Browse this author | Kawamura, Yoshimi Browse this author →KAKEN DB | Oka, Kaori Browse this author | Baghdadi, Muhammad Browse this author →KAKEN DB | Atsumi, Tatsuya Browse this author →KAKEN DB | Miura, Kyoko Browse this author | Seino, Ken-ichiro Browse this author →KAKEN DB |
Issue Date: | 29-Nov-2019 |
Publisher: | Nature Publishing Group |
Journal Title: | Scientific reports |
Volume: | 9 |
Start Page: | 17981 |
Publisher DOI: | 10.1038/s41598-019-54442-1 |
Abstract: | Naked mole rats (NMRs) have extraordinarily long lifespans and anti-tumorigenic capability. Recent studies of humans and mice have shown that many age-related diseases, including cancer, are strongly correlated with immunity, and macrophages play particularly important roles in immune regulation. Therefore, NMR macrophages may contribute to their unique phenotypes. However, studies of the roles of macrophages are limited by material restrictions and the lack of an established experimental strategy. In this study, we developed a flow cytometric strategy to identify NMR macrophages. The NMR macrophages were extractable using an off-the-shelf anti-CD11b antibody, M1/70, and forward/side scatter data obtained by flow cytometry. NMR macrophages proliferated in response to human/mouse recombinant M-CSF and engulfed Escherichia coli particles. Interestingly, the majority of NMR macrophages exhibited co-staining with an anti-NK1.1 antibody, PK136. NK1.1 antigen crosslinking with PK136 results in mouse NK cell stimulation; similarly, NMR macrophages proliferated in response to NK1.1 antibody treatment. Furthermore, we successfully established an NMR macrophage cell line, NPM1, by transduction of Simian virus 40 early region that proliferated indefinitely without cytokines and retained its phagocytotic capacity. The NPM1 would contribute to further studies on the immunity of NMRs. |
Rights: | https://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/76504 |
Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|