| Title: | Kinesin family members KIF11 and KIF23 as potential therapeutic targets in malignant pleural mesothelioma. |
| Authors: | Kato, Tatsuya Browse this author |
| Lee, Daiyoon Browse this author |
| Wu, Licun Browse this author |
| Patel, Priya Browse this author |
| Young, Ahn Jin Browse this author |
| Wada, Hironobu Browse this author →KAKEN DB |
| Hu, Hsin-Pei Browse this author |
| Ujiie, Hideki Browse this author |
| Kaji, Mitsuhito Browse this author →KAKEN DB |
| Kano, Satoshi Browse this author →KAKEN DB |
| Matsuge, Shinichi Browse this author |
| Domen, Hiromitsu Browse this author |
| Kaga, Kichizo Browse this author →KAKEN DB |
| Matsui, Yoshiro Browse this author →KAKEN DB |
| Kanno, Hiromi Browse this author |
| Hatanaka, Yutaka Browse this author →KAKEN DB |
| Hatanaka, Kanako C. Browse this author |
| Matsuno, Yoshihiro Browse this author →KAKEN DB |
| de Perrot, Marc Browse this author |
| Yasufuku, Kazuhiro Browse this author →KAKEN DB |
| Keywords: | KIF11 (Eg5) |
| KIF23 (MKLP1) |
| therapeutic target genes |
| immunohistochemistry |
| malignant pleural mesothelioma |
| Issue Date: | Aug-2016 |
| Publisher: | D.A. Spandidos |
| Journal Title: | International journal of oncology |
| Volume: | 49 |
| Issue: | 2 |
| Start Page: | 448 |
| End Page: | 456 |
| Publisher DOI: | 10.3892/ijo.2016.3566 |
| PMID: | 27279560 |
| Abstract: | Malignant pleural mesothelioma (MPM) is a rare and aggressive form of cancer commonly associated with asbestos exposure that stems from the thoracic mesothelium with high mortality rate. Currently, treatment options for MPM are limited, and new molecular targets for treatments are urgently needed. Using quantitative reverse transcription-polymerase chain reaction (RT-PCR) and an RNA interference-based screening, we screened two kinesin family members as potential therapeutic targets for MPM. Following in vitro investigation of the target silencing effects on MPM cells, a total of 53 MPMs were analyzed immunohistochemically with tissue microarray. KIF11 and KIF23 transcripts were found to be overexpressed in the majority of clinical MPM samples as well as human MPM cell lines as determined by quantitative RT-PCR. Gene knockdown in MPM cell lines identified growth inhibition following knockdown of KIF11 and KIF23. High expression of KIF11 (KIF11-H) and KIF23 (KIF23-H) were found in 43.4 and 50.9% of all the MPM cases, respectively. Patients who received curative resection with tumors displaying KIF23-H showed shorter overall survival (P=0.0194). These results provide that inhibition of KIF11 and KIF23 may hold promise for treatment of MPMs, raising the possibility that kinesin-based drug targets may be developed in the future. |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/76528 |
| Appears in Collections: | 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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