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Rabbit VX2 lung tumor models can form early nodal metastases

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Title: Rabbit VX2 lung tumor models can form early nodal metastases
Authors: Gregor, Alexander Browse this author
Fujino, Kosuke Browse this author
Bernards, Nicholas Browse this author
Kinoshita, Tomonari Browse this author
Motooka, Yamato Browse this author
Inage, Terunaga Browse this author
Ishiwata, Tsukasa Browse this author
Chen, Zhenchian Browse this author
Ujiie, Hideki Browse this author
Lee, Chang Young Browse this author
Yasufuku, Kazuhiro Browse this author →KAKEN DB
Keywords: Disease models
Animal
Rabbits
Nodal metastasis
Lung cancer
Issue Date: Dec-2019
Publisher: BioMed Central Ltd
Journal Title: World Journal of Surgical Oncology
Volume: 17
Issue: 1
Start Page: 231
Publisher DOI: 10.1186/s12957-019-1774-6
Abstract: Background: The rabbit squamous cell cancer line, VX2, has been used to generate various tumor models in rabbits. It is notable for its ability to generate nodal metastases. However, the timing and extent of nodal metastases vary by primary inoculation site and methodology. The development of metastases specifically in lung cancer models has not been well-described. We sought to characterize the generation of nodal metastases in rabbit transbronchial VX2 lung tumor models. Methods: Rabbit VX2 lung tumor models were created in the right lung via transbronchial injection and serially imaged by computed tomography. Rabbits (n = 15) were sacrificed from between 5 and 24 days post-inoculation for collection of the ipsilateral and contralateral paratracheal lymph nodes. These underwent histopathological evaluation for metastases using hematoxylin and eosin as well as cytokeratin AE1/AE3 immunohistochemical staining. Results: Nodal metastases were detectable as early as 1 week after inoculation but were more prevalent with longer inoculation; all rabbits at > 2 weeks post-inoculation had nodal metastases. Contralateral metastases were in general seen later than ipsilateral metastases. Lymph node volume did not predict the likelihood of nodal metastases (p = 0.4 and p = 0.07 for ipsilateral and contralateral nodal metastases, respectively), but primary tumor volume was significantly associated with the likelihood of nodal metastases (p = 0.001 and p = 0.005 for ipsilateral and contralateral nodal metastases, respectively). Ipsilateral metastases were detectable at a tumor diameter of 1 cm; contralateral metastases were more variable but in general required a tumor diameter of 2 cm. Conclusions: Rabbit transbronchial VX2 lung tumor models generate nodal metastases relatively early after inoculation. These results suggest such models may be valuable tools in the investigation of novel therapeutic modalities relevant for the treatment of both early-stage and locally advanced lung cancer. Keywords: Disease models, Animal, Rabbits, Nodal metastasis, Lung cancer
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/76531
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 氏家 秀樹

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