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Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos

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Title: Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos
Authors: LI, Jianye Browse this author
MAEJI, Mana Browse this author
BALBOULA, Ahmed Zaky Browse this author
ABOELENAIN, Mansour Browse this author
FUJII, Takashi Browse this author
MORIYASU, Satoru Browse this author
BAI, Hanako Browse this author →KAKEN DB
KAWAHARA, Manabu Browse this author →KAKEN DB
TAKAHASHI, Masashi Browse this author →KAKEN DB
Keywords: Cathepsin B
Cow
Lysosome
Oocytes
Preimplantation embryos
Issue Date: 2019
Publisher: 日本繁殖生物学会
Journal Title: Journal of Reproduction and Development
Publisher DOI: 10.1262/jrd.2019-115
Abstract: Lysosomal cathepsin, in particular cathepsin B (CTSB), plays an important role in implantation, pregnancy, and embryonic development. However, little is known about the mechanism related to the dynamic status of lysosomal cathepsins in bovine oocytes and preimplantation embryos. In the present study, we investigated the dynamics of gene expression, activity, and immunolocalization of CTSB, as well as the activities of lysosome, in bovine oocytes and preimplantation embryos. After gene expression analysis of several cathepsin-related genes, transcript levels of CTSB, CTSD and CTSZ were highest in MII oocytes followed by a significant decrease from the 8-cell embryo stage. Activity of CTSB showed a significant increase in 1-cell and morula stage embryos. Lysosomal activity was also significant higher in 1-cell and morula stages, which was consistent with CTSB activities. However, immunolocalization of CTSB did not show the similar pattern of CTSB and lysosomal activities. We also found significantly higher expression levels of CTSB transcript in the trophectoderm (TE) compared to inner cell mass (ICM), whereas activity and immunolocalization of CTSB showed an opposite pattern, i.e. significantly higher in ICM than TE. These patterns were confirmed by the same analysis using separated ICM and TE. Our results suggest that lysosomal CTSB has a pivotal role during embryonic development and differentiation, especially fertilization and the differentiation period.
Rights: This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license. https://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/76590
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 昌志

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