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Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes

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Title: Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
Authors: LI, Jianye Browse this author
BALBOULA, Ahmed Zaky Browse this author
ABOELENAIN, Mansour Browse this author
FUJII, Takashi Browse this author
MORIYASU, Satoru Browse this author
BAI, Hanako Browse this author →KAKEN DB
KAWAHARA, Manabu Browse this author →KAKEN DB
TAKAHASHI, Masashi Browse this author →KAKEN DB
Keywords: Autophagy
Cathepsin B
Cow
Developmental competence
Oocyte quality
Issue Date: 2019
Publisher: 日本繁殖生物学会
Journal Title: Journal of Reproduction and Development
Publisher DOI: 10.1262/jrd.2019-123
Abstract: The present study investigated the effect of autophagy induction and cathepsin B (CTSB) inhibition on developmental competence of poor quality oocytes. Bovine cumulus oocyte complexes (COCs) were classified as good or poor according to their morphology. Autophagy activity was detected in good and poor germinal vesicle (GV) oocytes. Then E-64, a CTSB inhibitor, rapamycin (Rapa), an autophagy inducer, and combined administration was achieved during in vitro maturation (IVM) of poor quality COCs followed by detection of autophagy activity. In the next experiment, E-64, Rapa, and E64 + Rapa, were added during IVM to good and poor quality COCs followed by in vitro fertilization and culture for 8 days to investigate whether inhibition of CTSB and/or induction of autophagy improve embryonic development and quality. Autophagy activity was significantly lower in poor quality GV oocytes than in good quality ones. E-64, Rapa and E-64 + Rapa treatment during IVM significantly increased autophagy activity in poor quality oocytes. Addition of Rapa in good quality COCs did not increase the blastocyst rate, whereas E-64 increased the blastocyst rate and total cell number (TCN) with decreasing TUNEL-positive cells. In contrast, Rapa treatment in poor quality COCs significantly increased the blastocyst rate and TCN with decreasing TUNEL-positive cells. These results indicate oocyte quality has different responses to intracellular autophagy induction and CTSB activity control by potential autophagy and catabolic status, however, synergetic effect of autophagy induction and CTSB inhibition can increase developmental competence of both good and poor quality COCs, especially rescue effect in poor quality COCs.
Rights: This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license. https://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/76591
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 昌志

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