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Hokkaido University Collection of Scholarly and Academic Papers >
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Hot topic: Pregnancy-induced expression of interferon-stimulated genes in the cervical and vaginal mucosal membranes
| Title: | Hot topic: Pregnancy-induced expression of interferon-stimulated genes in the cervical and vaginal mucosal membranes |
| Authors: | Kunii, Hiroki Browse this author | | Koyama, Keisuke Browse this author | | Ito, Tsukino Browse this author | | Suzuki, Toshiyuki Browse this author | | Balboula, Ahmed Z Browse this author | | Shirozu, Takahiro Browse this author | | Bai, Hanako Browse this author →KAKEN DB | | Nagano, Masashi Browse this author →KAKEN DB | | Kawahara, Manabu Browse this author →KAKEN DB | | Takahashi, Masashi Browse this author →KAKEN DB |
| Keywords: | pregnancy | | interferon-stimulated genes | | cervix | | vagina |
| Issue Date: | Sep-2018 |
| Publisher: | American Dairy Science Association |
| Journal Title: | Journal of Dairy Science |
| Volume: | 101 |
| Issue: | 9 |
| Start Page: | 8396 |
| End Page: | 8400 |
| Publisher DOI: | 10.3168/jds.2017-14251 |
| Abstract: | In ruminants, IFN-tau (IFNT) is a pregnancy recognition signal secreted by the embryonic trophectoderm before implantation, and it induces the expression of IFN-stimulated genes (ISG) in the uterine endometrium and blood leukocytes. The expression of ISG in blood leukocytes could indicate the presence of a viable conceptus before return of the next estrus; however, expression levels have high variation for confirming pregnancy. We hypothesized that the secreted IFNT in the uterus would affect ISG expression in cervical and vaginal tissues because they are directly adjacent to the uterus. To prove the hypothesis, we investigated the expression of 3 ISG (ISG15, MX1, and MX2) in cervical and vaginal mucosal membranes collected from pregnant (n = 12) and nonpregnant (n = 11) lactating Holstein cows at 17 to 18 d after artificial insemination. Mucosal membrane samples of the cervical canal near the external os (cervix) and deep vaginal wall surrounding the external os (vagina) were collected separately by simply scraping with a curette on d 17 or 18 of pregnancy (d 1 = ovulation), at which time IFNT secretion into the maternal uterus is maximal. After pregnancy diagnosis on d 30 and 60, separately collected samples confirmed as pregnant and nonpregnant were used for evaluation of the expression of IFN-stimulated protein 15 kDa (ISG15) and myxovirus-resistance protein 1 and 2 (MX1, MX2) with quantitative real-time PCR. The collected mucosal membrane samples from cervix contained mostly cell clots showing membrane structure and a low content of blood cells. The expression levels of all 3 genes were significantly increased in pregnant cows compared with nonpregnant cows in both cervical and vaginal samples. These results suggest that increased expression of ISG in the cervix and vagina is a pregnancy-associated phenomenon and is highly affected by IFNT secreted from the conceptus through the uterus. |
| Rights: | © 2018 American Dairy Science Association®., Open Archive in partnership with American Dairy Science Association (ADSA) Under an Elsevier user license. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/76595 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高橋 昌志
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