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Hot topic: Pregnancy-induced expression of interferon-stimulated genes in the cervical and vaginal mucosal membranes

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Title: Hot topic: Pregnancy-induced expression of interferon-stimulated genes in the cervical and vaginal mucosal membranes
Authors: Kunii, Hiroki Browse this author
Koyama, Keisuke Browse this author
Ito, Tsukino Browse this author
Suzuki, Toshiyuki Browse this author
Balboula, Ahmed Z Browse this author
Shirozu, Takahiro Browse this author
Bai, Hanako Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Takahashi, Masashi Browse this author →KAKEN DB
Keywords: pregnancy
interferon-stimulated genes
Issue Date: Sep-2018
Publisher: American Dairy Science Association
Journal Title: Journal of Dairy Science
Volume: 101
Issue: 9
Start Page: 8396
End Page: 8400
Publisher DOI: 10.3168/jds.2017-14251
Abstract: In ruminants, IFN-tau (IFNT) is a pregnancy recognition signal secreted by the embryonic trophectoderm before implantation, and it induces the expression of IFN-stimulated genes (ISG) in the uterine endometrium and blood leukocytes. The expression of ISG in blood leukocytes could indicate the presence of a viable conceptus before return of the next estrus; however, expression levels have high variation for confirming pregnancy. We hypothesized that the secreted IFNT in the uterus would affect ISG expression in cervical and vaginal tissues because they are directly adjacent to the uterus. To prove the hypothesis, we investigated the expression of 3 ISG (ISG15, MX1, and MX2) in cervical and vaginal mucosal membranes collected from pregnant (n = 12) and nonpregnant (n = 11) lactating Holstein cows at 17 to 18 d after artificial insemination. Mucosal membrane samples of the cervical canal near the external os (cervix) and deep vaginal wall surrounding the external os (vagina) were collected separately by simply scraping with a curette on d 17 or 18 of pregnancy (d 1 = ovulation), at which time IFNT secretion into the maternal uterus is maximal. After pregnancy diagnosis on d 30 and 60, separately collected samples confirmed as pregnant and nonpregnant were used for evaluation of the expression of IFN-stimulated protein 15 kDa (ISG15) and myxovirus-resistance protein 1 and 2 (MX1, MX2) with quantitative real-time PCR. The collected mucosal membrane samples from cervix contained mostly cell clots showing membrane structure and a low content of blood cells. The expression levels of all 3 genes were significantly increased in pregnant cows compared with nonpregnant cows in both cervical and vaginal samples. These results suggest that increased expression of ISG in the cervix and vagina is a pregnancy-associated phenomenon and is highly affected by IFNT secreted from the conceptus through the uterus.
Rights: © 2018 American Dairy Science Association®., Open Archive in partnership with American Dairy Science Association (ADSA) Under an Elsevier user license.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 昌志

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