Title: | Ki67 expression and localization of T cells after neoadjuvant therapies as reliable predictive markers in rectal cancer |
Authors: | Imaizumi, Ken Browse this author |
Suzuki, Toshihiro Browse this author |
Kojima, Motohiro Browse this author →KAKEN DB |
Shimomura, Manami Browse this author |
Sakuyama, Naoki Browse this author |
Tsukada, Yuichiro Browse this author |
Sasaki, Takeshi Browse this author |
Nishizawa, Yuji Browse this author →KAKEN DB |
Taketomi, Akinobu Browse this author →KAKEN DB |
Ito, Masaaki Browse this author →KAKEN DB |
Nakatsura, Tetsuya Browse this author →KAKEN DB |
Keywords: | multiplexed fluorescent immunohistochemistry |
neoadjuvant chemoradiotherapy |
neoadjuvant chemotherapy |
rectal cancer |
tumor-infiltrating lymphocyte |
Issue Date: | Jan-2020 |
Publisher: | John Wiley & Sons |
Journal Title: | Cancer science |
Volume: | 111 |
Issue: | 1 |
Start Page: | 23 |
End Page: | 35 |
Publisher DOI: | 10.1111/cas.14223 |
Abstract: | Chemoradiotherapy (CRT) is the standard neoadjuvant therapy for locally advanced rectal cancer (RC). However, neoadjuvant chemotherapy (NAC) also shows favorable outcomes. Although the immunological environment of RC has been thoroughly discussed, the effect of NAC on it is less clear. Here, we investigated the immunological microenvironment, including T cell infiltration, activation, and topological distribution, of resected RC tissue after neoadjuvant therapies and evaluated the correlation between T cell subsets and patient prognosis. Rectal cancer patients (n = 188) were enrolled and categorized into 3 groups, namely CRT (n = 41), NAC (n = 46), and control (surgery alone; n = 101) groups. Characterization of residual carcinoma cells and T cell subsets in resected tissues was performed using multiplex fluorescence immunohistochemistry. The densities of total and activated (Ki67(high)) T cells in tissues after NAC, but not CRT, were higher than in control. In both CRT and NAC groups, patients presenting with higher treatment effects showed aggressive infiltration of T cell subsets into carcinomas. Multivariate analyses of pathological and immunological features and prognosis revealed that carcinoma Ki67(high)CD4(+) T cells after CRT and stromal Ki67(high)CD8(+) T cells after NAC are important prognostic factors, respectively. Our results suggest that evaluation of T cell activation with Ki67 expression and its tumor localization can be used to determine the prognosis of advanced RC after neoadjuvant therapies. |
Rights: | http://creativecommons.org/licenses/by-nc/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/76616 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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