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Exogenous Expression of Equine MHC Class I Molecules in Mice Increases Susceptibility to Equine Herpesvirus 1 Pulmonary Infection

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/76630

Title: Exogenous Expression of Equine MHC Class I Molecules in Mice Increases Susceptibility to Equine Herpesvirus 1 Pulmonary Infection
Authors: Minato, Erina Browse this author
Aoshima, Keisuke Browse this author
Kobayashi, Atsushi Browse this author
Ohnishi, Naomi Browse this author
Sasaki, Nobuya Browse this author
Kimura, Takashi Browse this author →KAKEN DB
Keywords: equine herpesvirus 1
MHC class I
transgenic mice
horses
respiratory diseases
nervous system diseases
Issue Date: Sep-2019
Publisher: SAGE Publications
Journal Title: Veterinary pathology
Volume: 56
Issue: 5
Start Page: 703
End Page: 710
Publisher DOI: 10.1177/0300985819834616
PMID: 30866742
Abstract: Equine herpesvirus 1 (EHV-1) uses equine major histocompatibility complex class I (MHC class I) as an entry receptor. Exogenous expression of equine MHC class I genes in murine cell lines confers susceptibility to EHV-1 infection. To examine the in vivo role of equine MHC class I as an entry receptor for EHV-1, we generated transgenic (Tg) mice expressing equine MHC class I under the control of the CAG promoter. Equine MHC class I protein was expressed in the liver, spleen, lung, and brain of Tg mice, which was confirmed by Western blot. However, equine MHC class I antigen was only detected in bronchiolar epithelium and not in other tissues, using the immunofluorescence method employed in this study. Both Tg and wild-type (WT) mice developed pneumonia 3 days after intranasal infection with EHV-1. The bronchiolar epithelial cells of Tg mice showed more severe necrosis, compared with those in WT mice. In addition, the number of virus antigen-positive cells in the lungs was higher in Tg mice than in WT mice. These results suggest that exogenous expression of equine MHC class I renders mice more susceptible to EHV-1 infection.
Rights: Erina Minato, Keisuke Aoshima, Atsushi Kobayashi, Naomi Ohnishi, Nobuya Sasaki, Takashi Kimura, Exogenous Expression of Equine MHC Class I Molecules in Mice Increases Susceptibility to Equine Herpesvirus 1 Pulmonary Infection, Veterinary Pathology (Vol 56 Issue 5, 2019) pp. 703-710. Copyright © 2019 (The Authors). DOI: 10.1177/0300985819834616.
Type: article (author version)
URI: http://hdl.handle.net/2115/76630
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 港 江利奈

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