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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Exogenous Expression of Equine MHC Class I Molecules in Mice Increases Susceptibility to Equine Herpesvirus 1 Pulmonary Infection
| Title: | Exogenous Expression of Equine MHC Class I Molecules in Mice Increases Susceptibility to Equine Herpesvirus 1 Pulmonary Infection |
| Authors: | Minato, Erina Browse this author | | Aoshima, Keisuke Browse this author | | Kobayashi, Atsushi Browse this author | | Ohnishi, Naomi Browse this author | | Sasaki, Nobuya Browse this author | | Kimura, Takashi Browse this author →KAKEN DB |
| Keywords: | equine herpesvirus 1 | | MHC class I | | transgenic mice | | horses | | respiratory diseases | | nervous system diseases |
| Issue Date: | Sep-2019 |
| Publisher: | SAGE Publications |
| Journal Title: | Veterinary pathology |
| Volume: | 56 |
| Issue: | 5 |
| Start Page: | 703 |
| End Page: | 710 |
| Publisher DOI: | 10.1177/0300985819834616 |
| PMID: | 30866742 |
| Abstract: | Equine herpesvirus 1 (EHV-1) uses equine major histocompatibility complex class I (MHC class I) as an entry receptor. Exogenous expression of equine MHC class I genes in murine cell lines confers susceptibility to EHV-1 infection. To examine the in vivo role of equine MHC class I as an entry receptor for EHV-1, we generated transgenic (Tg) mice expressing equine MHC class I under the control of the CAG promoter. Equine MHC class I protein was expressed in the liver, spleen, lung, and brain of Tg mice, which was confirmed by Western blot. However, equine MHC class I antigen was only detected in bronchiolar epithelium and not in other tissues, using the immunofluorescence method employed in this study. Both Tg and wild-type (WT) mice developed pneumonia 3 days after intranasal infection with EHV-1. The bronchiolar epithelial cells of Tg mice showed more severe necrosis, compared with those in WT mice. In addition, the number of virus antigen-positive cells in the lungs was higher in Tg mice than in WT mice. These results suggest that exogenous expression of equine MHC class I renders mice more susceptible to EHV-1 infection. |
| Rights: | Erina Minato, Keisuke Aoshima, Atsushi Kobayashi, Naomi Ohnishi, Nobuya Sasaki, Takashi Kimura, Exogenous Expression of Equine MHC Class I Molecules in Mice Increases Susceptibility to Equine Herpesvirus 1 Pulmonary Infection, Veterinary Pathology (Vol 56 Issue 5, 2019) pp. 703-710. Copyright © 2019 (The Authors). DOI: 10.1177/0300985819834616. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/76630 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 港 江利奈
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