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The pH-dependent conformational change of eukaryotic translation initiation factor 5: Insights into partner-binding manner

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Title: The pH-dependent conformational change of eukaryotic translation initiation factor 5: Insights into partner-binding manner
Authors: Ye, Yuxin Browse this author
Chen, Meirong Browse this author
Kato, Koji Browse this author
Yao, Min Browse this author →KAKEN DB
Keywords: elF5
AA-box
eIF2β
K-box
Structure
Issue Date: 29-Oct-2019
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 519
Issue: 1
Start Page: 186
End Page: 191
Publisher DOI: 10.1016/j.bbrc.2019.08.128
Abstract: In the process of eukaryotic translation, the formation of preinitiation complex 43S, which consists of a 40S subunit, the eIF2-GTP-Met-tRNAi(Met) ternary complex, eIF3, eIF1, eIF1A, and eIF5, is essential for translational quality control. Of those factors, eIF5 promotes the hydrolysis of eIF2-bound GTP to release eIF2-GDP in the complex for the recycling of eIF2. eIF5 appears to bind to the beta subunit of eIF2 (eIF2 beta) via an interaction between aromatic/acidic residue-rich regions (AA-boxes) in the C-terminal domain of eIF5 (eIF5CTD) and three lysine clusters (K-boxes) in the N-terminal domain of eIF2 beta (eIF2 beta NTD). However, the details of this interaction are unclear, due to the lack of a structure for the eIF5-eIF2 beta complex, and the unavailability of an intact structure of eIF5, in which the AA-boxes are always disordered, with high flexibility. In this study, we solved two crystal structures of eIF5CTD from Candida albicans, which for the first time showed the AA-box2 of eIF5 presenting as an ordered helical structure. The structures exhibited different arrangements of AA-box2 under different pH values, which may reflect the dynamic nature of the interactions of eIF5CTD, and eIF2 beta NTD in the preinitiation complex. (C) 2019 The Authors. Published by Elsevier Inc.
Rights: http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/76740
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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