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Feasibility of pedicled vascularized inguinal lymph node transfer in a mouse model : A preliminary study
| Title: | Feasibility of pedicled vascularized inguinal lymph node transfer in a mouse model : A preliminary study |
| Authors: | Ishikawa, Kosuke Browse this author →KAKEN DB | | Maeda, Taku Browse this author | | Funayama, Emi Browse this author | | Hayashi, Toshihiko Browse this author →KAKEN DB | | Murao, Naoki Browse this author →KAKEN DB | | Osawa, Masayuki Browse this author | | Furukawa, Hiroshi Browse this author →KAKEN DB | | Oyama, Akihiko Browse this author | | Yamamoto, Yuhei Browse this author →KAKEN DB |
| Issue Date: | Mar-2019 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Microsurgery |
| Volume: | 39 |
| Issue: | 3 |
| Start Page: | 247 |
| End Page: | 254 |
| Publisher DOI: | 10.1002/micr.30394 |
| PMID: | 30508286 |
| Abstract: | PurposeVascularized lymph node transfer is becoming more common in the treatment of lymphedema, but suitable small animal models for research are lacking. Here, we evaluated the feasibility of pedicled vascularized inguinal lymph node transfer in mice. MethodsTwenty-five mice were used in the study. An inguinal lymph node-bearing flap with a vascular pedicle containing the superficial caudal epigastric vessels was transferred into the ipsilateral popliteal fossa after excision of the popliteal lymph node. Indocyanine green (ICG) angiography was used to confirm vascularity of the flap. ICG lymphography was performed to evaluate lymphatic flow at 3 and 4 weeks postoperatively. Patent blue dye was injected into the ipsilateral hind paw to observe staining of the transferred lymph node at 4 weeks postoperatively. All transferred lymph nodes were then harvested and histologically evaluated by hematoxylin and eosin staining. ResultsIn 16 of the 25 mice, ICG lymphography showed reconnection between the transferred lymph node and the afferent lymphatic vessels, as confirmed by patent blue staining. Histologically, these transferred lymph nodes with afferent lymphatic reconnection significantly regressed in size (0.370.24mm(2)) and showed clear follicle formation, whereas those without afferent lymphatic reconnection showed less size regression (1.31 +/- 1.17mm(2)); the cell population was too dense to allow identification of follicles. ConclusionsWe established a mouse model of vascularized lymph node transfer with predictable afferent lymphatic reconnection. Both the vascularization and reconnection might be necessary for functional regeneration of the transferred lymph node. |
| Rights: | This is the peer reviewed version of the following article: Microsurgery. 2019, 39(3), 247-254, which has been published in final form at https://doi.org/10.1002/micr.30394. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/76825 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石川 耕資
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