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Cell type-specific reciprocal regulation of HIF1A gene expression is dependent on 5′- and 3′-UTRs
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| Title: | Cell type-specific reciprocal regulation of HIF1A gene expression is dependent on 5′- and 3′-UTRs |
| Authors: | Yasuda, Motoaki Browse this author →KAKEN DB | | Hatanaka, Tomoyuki Browse this author | | Shirato, Hiroki Browse this author →KAKEN DB | | Nishioka, Takeshi Browse this author →KAKEN DB |
| Keywords: | Hypoxia | | HIF-1α | | UTR | | Translation | | Transcription |
| Issue Date: | 16-May-2014 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 447 |
| Issue: | 4 |
| Start Page: | 638 |
| End Page: | 643 |
| Publisher DOI: | 10.1016/j.bbrc.2014.04.058 |
| PMID: | 24769203 |
| Abstract: | In the present study, we demonstrated the reciprocal regulation of hypoxia-inducible factor 1 alpha (HIF1A) gene expression via untranslated region-(UTR) dependent mechanisms. A 151 nucleotide sequence found in the HIF1A 5′-UTR is sufficient for significant translational up-regulation. On the other hand, the 3′-UTR of HIF1A has been implicated in mRNA degradation. In the non-metastatic breast cancer cell line MCF7, the 3′-UTR-dependent down-regulatory machinery predominates over the 5′-UTR-dependent up-regulation of HIF1A. However, 5′-UTR-dependent up-regulation is dominant among metastatic cell lines (MDA-MB453, U87MG). It is therefore likely that the predominance of 5′-UTR-dependent translational enhancement of HIF1A is critical for the malignant phenotype of cancer cells. PTBP-1, but not HuR, is a candidate RNA binding protein for the translational control of HIF1A. |
| Rights: | © <2014>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/76837 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 安田 元昭
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