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A domain responsible for spontaneous conversion of bank vole prion protein

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/76862

Title: A domain responsible for spontaneous conversion of bank vole prion protein
Authors: Kobayashi, Atsushi Browse this author
Matsuura, Yuichi Browse this author →KAKEN DB
Takeuchi, Atsuko Browse this author →KAKEN DB
Yamada, Masahito Browse this author →KAKEN DB
Miyoshi, Ichiro Browse this author →KAKEN DB
Mohri, Shirou Browse this author →KAKEN DB
Kitamoto, Tetsuyuki Browse this author →KAKEN DB
Keywords: prion
prion disease
bank vole
misfolding
Issue Date: Mar-2019
Publisher: John Wiley & Sons
Journal Title: Brain pathology
Volume: 29
Issue: 2
Start Page: 155
End Page: 163
Publisher DOI: 10.1111/bpa.12638
Abstract: Bank vole is a small rodent that shows high susceptibility to infection with diverse prion strains. To determine whether the increased susceptibility of bank voles to prion diseases can be attributed to the intrinsic nature of bank vole prion protein (PrP) or to host factors other than PrP, we produced transgenic mice overexpressing bank vole PrP. These transgenic mice spontaneously developed neurological illness with spongiform changes and the accumulation of abnormal PrP in the brain. Then, we produced transgenic mice overexpressing chimeric mouse/bank vole PrP, which differs from mouse PrP only at two residues located at the C-terminus, to determine the minimum essential domain for the induction of spontaneous generation of abnormal PrP. These transgenic mice also developed spontaneous neurological illness with spongiform changes and the accumulation of abnormal PrP in the brain. In addition, knock-in mice expressing bank vole PrP at the same level as that of wild-type mice did not develop spontaneous disease but showed high susceptibility to infection with diverse prion strains, similarly to bank voles. Taken together, these findings show that bank vole PrP has a high propensity for the conformational conversion both in spontaneous disease and in prion infection, probably due to the characteristic structural properties of the C-terminal domain.
Rights: This is the peer reviewed version of the following article: Atsushi K. et al, "A domain responsible for spontaneous conversion of bank vole prion protein, which has been published in final form at https://doi.org/10.1111/bpa.12638. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
URI: http://hdl.handle.net/2115/76862
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 篤史

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