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Efficient isolation of Swine influenza viruses by age-targeted specimen collection

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Title: Efficient isolation of Swine influenza viruses by age-targeted specimen collection
Authors: Ozawa, Makoto1 Browse this author →KAKEN DB
Matsuu, Aya2 Browse this author →KAKEN DB
Yonezawa, Kouki3 Browse this author →KAKEN DB
Igarashi, Manabu4 Browse this author →KAKEN DB
Okuya, Kosuke5 Browse this author
Kawabata, Toshiko6 Browse this author
Ito, Kimihito7 Browse this author →KAKEN DB
Kohara, Kyoko8 Browse this author →KAKEN DB
Taneno, Akira9 Browse this author
Deguchi, Eisaburo10 Browse this author
Authors(alt): Tsukiyama, Kyoko8
Issue Date: Apr-2015
Publisher: American Society for Microbiology
Journal Title: Journal Clinical Microbiology
Volume: 53
Issue: 4
Start Page: 1331
End Page: 1338
Publisher DOI: 10.1128/JCM.02941-14
PMID: 25694523
Abstract: The control of swine influenza virus (SIV) infection is paramount for increasing the productivity of pig farming and minimizing the threat of pandemic outbreaks. Thus, SIV surveillance should be conducted by region and on a regular basis. Here, we established a microneutralization assay specific for SIV seroprevalence surveillance by using reporter gene-expressing recombinant influenza viruses. Growth-based SIV seroprevalence revealed that most sows and piglets were positive for neutralizing antibodies against influenza viruses. In contrast, the 90-day-old growing pigs exhibited limited neutralizing activity in their sera, suggesting that this particular age of population is most susceptible to SIV infection and thus is an ideal age group for SIV isolation. From nasal swab specimens of healthy pigs in this age population, we were able to isolate SIVs at a higher incidence (5.3%) than those of previous reports. Nucleotide sequencing and phylogenetic analysis of the hemagglutinin (HA) genes revealed that the isolated SIVs have circulated and evolved in pigs but not have been recently introduced from humans, implying that a large number of SIV lineages may remain “undiscovered” in the global porcine populations. We propose that the 90-day-old growing pig-targeted nasal swab collection presented in this study facilitates global SIV surveillance and contributes to the detection and control of SIV infection.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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