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Efficient isolation of Swine influenza viruses by age-targeted specimen collection
Title: | Efficient isolation of Swine influenza viruses by age-targeted specimen collection |
Authors: | Ozawa, Makoto1 Browse this author →KAKEN DB | Matsuu, Aya2 Browse this author →KAKEN DB | Yonezawa, Kouki3 Browse this author →KAKEN DB | Igarashi, Manabu4 Browse this author →KAKEN DB | Okuya, Kosuke5 Browse this author | Kawabata, Toshiko6 Browse this author | Ito, Kimihito7 Browse this author →KAKEN DB | Kohara, Kyoko8 Browse this author →KAKEN DB | Taneno, Akira9 Browse this author | Deguchi, Eisaburo10 Browse this author |
Authors(alt): | Tsukiyama, Kyoko8 |
Issue Date: | Apr-2015 |
Publisher: | American Society for Microbiology |
Journal Title: | Journal Clinical Microbiology |
Volume: | 53 |
Issue: | 4 |
Start Page: | 1331 |
End Page: | 1338 |
Publisher DOI: | 10.1128/JCM.02941-14 |
PMID: | 25694523 |
Abstract: | The control of swine influenza virus (SIV) infection is paramount for increasing the productivity of pig farming and minimizing the threat of pandemic outbreaks. Thus, SIV surveillance should be conducted by region and on a regular basis. Here, we established a microneutralization assay specific for SIV seroprevalence surveillance by using reporter gene-expressing recombinant influenza viruses. Growth-based SIV seroprevalence revealed that most sows and piglets were positive for neutralizing antibodies against influenza viruses. In contrast, the 90-day-old growing pigs exhibited limited neutralizing activity in their sera, suggesting that this particular age of population is most susceptible to SIV infection and thus is an ideal age group for SIV isolation. From nasal swab specimens of healthy pigs in this age population, we were able to isolate SIVs at a higher incidence (5.3%) than those of previous reports. Nucleotide sequencing and phylogenetic analysis of the hemagglutinin (HA) genes revealed that the isolated SIVs have circulated and evolved in pigs but not have been recently introduced from humans, implying that a large number of SIV lineages may remain “undiscovered” in the global porcine populations. We propose that the 90-day-old growing pig-targeted nasal swab collection presented in this study facilitates global SIV surveillance and contributes to the detection and control of SIV infection. |
Type: | article |
URI: | http://hdl.handle.net/2115/76879 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc) 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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