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First report of Klebsiella quasipneumoniae harboring bla(KPC-2) in Saudi Arabia
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Title: | First report of Klebsiella quasipneumoniae harboring bla(KPC-2) in Saudi Arabia |
Authors: | Hala, Sharif Browse this author | Antony, Chakkiath Paul Browse this author | Alshehri, Mohammed Browse this author | Althaqafi, O. Abdulhakeem Browse this author | Alsaedi, Asim Browse this author | Mufti, Areej Browse this author | Kaaki, Mai Browse this author | Alhaj-Hussein, T. Baraa Browse this author | Zowawi, M. Hosam Browse this author | Al-Amri, Abdulfattah Browse this author | Pain, Arnab Browse this author |
Keywords: | MDR | Klebsiella quasipneumoniae | Carbapenemases | KPC-2 | bla(KPC-2) | Tn3 |
Issue Date: | 19-Dec-2019 |
Publisher: | BioMed Central |
Journal Title: | Antimicrobial resistance and infection control |
Volume: | 8 |
Issue: | 1 |
Start Page: | 203 |
Publisher DOI: | 10.1186/s13756-019-0653-9 |
PMID: | 31890159 |
Abstract: | Background Nosocomial infections caused by multi-drug resistant Enterobacteriaceae are a global public health threat that ought to be promptly identified, reported, and addressed accurately. Many carbapenem-resistant Enterobacteriaceae-associated genes have been identified in Saudi Arabia but not the endemic Klebsiella pneumoniae carbapenemases (KPCs), which are encoded by bla(KPC-type) genes. KPCs are known for their exceptional spreading potential. Methods We collected n = 286 multi-drug resistant (MDR) Klebsiella spp. isolates as part of screening for resistant patterns from a tertiary hospital in Saudi Arabia between 2014 and 2018. Antimicrobial susceptibility testing was carried out using both VITEK II and the broth microdilution of all collected isolates. Detection of resistance-conferring genes was carried out using Illumina whole-genome shotgun sequencing and PacBio SMRT sequencing protocols. Results A Carbapenem-resistant Enterobacteriaceae (CRE) Klebsiella quasipneumoniae subsp. similipneumoniae strain was identified as a novel ST-3510 carrying a bla(KPC-2) carbapenemase encoding gene. The isolate, designated as NGKPC-421, was obtained from shotgun Whole Genome Sequencing (WGS) surveillance of 286 MDR Klebsiella spp. clinical isolates. The NGKPC-421 isolate was collected from a septic patient in late 2017 and was initially misidentified as K. pneumoniae. The sequencing and assembly of the NGKPC-421 genome resulted in the identification of a putative similar to 39.4 kb IncX6 plasmid harboring a bla(KPC-2) gene, flanked by transposable elements (ISKpn6-bla(KPC-2)-ISKpn27). Conclusion This is the first identification of a KPC-2-producing CRE in the Gulf region. The impact on this finding is of major concern to the public health in Saudi Arabia, considering that it is the religious epicenter with a continuous mass influx of pilgrims from across the world. Our study strongly highlights the importance of implementing rapid sequencing-based technologies in clinical microbiology for precise taxonomic classification and monitoring of antimicrobial resistance patterns. |
Rights: | https://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/76928 |
Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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