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First report of Klebsiella quasipneumoniae harboring bla(KPC-2) in Saudi Arabia

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Title: First report of Klebsiella quasipneumoniae harboring bla(KPC-2) in Saudi Arabia
Authors: Hala, Sharif Browse this author
Antony, Chakkiath Paul Browse this author
Alshehri, Mohammed Browse this author
Althaqafi, O. Abdulhakeem Browse this author
Alsaedi, Asim Browse this author
Mufti, Areej Browse this author
Kaaki, Mai Browse this author
Alhaj-Hussein, T. Baraa Browse this author
Zowawi, M. Hosam Browse this author
Al-Amri, Abdulfattah Browse this author
Pain, Arnab Browse this author
Keywords: MDR
Klebsiella quasipneumoniae
Issue Date: 19-Dec-2019
Publisher: BioMed Central
Journal Title: Antimicrobial resistance and infection control
Volume: 8
Issue: 1
Start Page: 203
Publisher DOI: 10.1186/s13756-019-0653-9
PMID: 31890159
Abstract: Background Nosocomial infections caused by multi-drug resistant Enterobacteriaceae are a global public health threat that ought to be promptly identified, reported, and addressed accurately. Many carbapenem-resistant Enterobacteriaceae-associated genes have been identified in Saudi Arabia but not the endemic Klebsiella pneumoniae carbapenemases (KPCs), which are encoded by bla(KPC-type) genes. KPCs are known for their exceptional spreading potential. Methods We collected n = 286 multi-drug resistant (MDR) Klebsiella spp. isolates as part of screening for resistant patterns from a tertiary hospital in Saudi Arabia between 2014 and 2018. Antimicrobial susceptibility testing was carried out using both VITEK II and the broth microdilution of all collected isolates. Detection of resistance-conferring genes was carried out using Illumina whole-genome shotgun sequencing and PacBio SMRT sequencing protocols. Results A Carbapenem-resistant Enterobacteriaceae (CRE) Klebsiella quasipneumoniae subsp. similipneumoniae strain was identified as a novel ST-3510 carrying a bla(KPC-2) carbapenemase encoding gene. The isolate, designated as NGKPC-421, was obtained from shotgun Whole Genome Sequencing (WGS) surveillance of 286 MDR Klebsiella spp. clinical isolates. The NGKPC-421 isolate was collected from a septic patient in late 2017 and was initially misidentified as K. pneumoniae. The sequencing and assembly of the NGKPC-421 genome resulted in the identification of a putative similar to 39.4 kb IncX6 plasmid harboring a bla(KPC-2) gene, flanked by transposable elements (ISKpn6-bla(KPC-2)-ISKpn27). Conclusion This is the first identification of a KPC-2-producing CRE in the Gulf region. The impact on this finding is of major concern to the public health in Saudi Arabia, considering that it is the religious epicenter with a continuous mass influx of pilgrims from across the world. Our study strongly highlights the importance of implementing rapid sequencing-based technologies in clinical microbiology for precise taxonomic classification and monitoring of antimicrobial resistance patterns.
Type: article
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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