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博士 （歯学） >
FUSION OF RAW 264.7 MACROPHAGE CELLS ON MICRO-SCALE FINE PILLAR PATTERNS
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| Title: | FUSION OF RAW 264.7 MACROPHAGE CELLS ON MICRO-SCALE FINE PILLAR PATTERNS |
| Other Titles: | マイクロスケールパターン上におけるRAW264.7細胞の融合 |
| Authors: | 本間, 淳 Browse this author |
| Keywords: | Cell attachment | | macrophage fusion | | micro-pattern | | pillar-pattern | | RAW 264.7 |
| Issue Date: | 25-Mar-2019 |
| Publisher: | Hokkaido University |
| Abstract: | Macrophage-dependent immune response to the implant surface sometimes results in failure of implant. Macrophage behaviors are sensitive to the topological factors involving micro/nano-patterns on the implant surface. However, further investigations about macrophage behaviors toward the fine micro/nano-patterns on the implant surface are required. In the present study, we investigated the effects of fine micro-scale pillar patterns found on the surface of implants on macrophage fusion. Micropillars with diameters of 2 μm, 1 μm, and 500 nm and a height of 500 nm were fabricated by nanoimprinting on a cyclo-olefin polymer (COP) film. RAW 264.7 macrophages attachment assay shows that 1 μm- and 500 nm-pillars improved cell attachment than the planar surface. Macrophage fusion assay shows that the number of multinucleated cells on 1 μm- and 500 nm-pillars was approximately 1.4 to 1.5 times higher than that on the planar surface. However, 2 μm-pillar was ineffective in both the assays. Interestingly, the expression of F-actin and vinculin in mononuclear and multinucleated cells on the pillars appeared ring shaped along the circumference of pillars, indicating frustrated phagocytosis. We suggest that fine micro-patterning of the substrate could be one of the factors that control cell attachment and fusion of RAW 264.7 cells. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第13477号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 教授 吉田 靖弘, 教授 網塚 憲生, 准教授 佐藤 嘉晃 |
| Degree Affiliation: | 歯学研究科(口腔医学専攻) |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/77059 |
| Appears in Collections: | 課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
学位論文 (Theses) > 博士 （歯学）
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