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Fabrication and characterization of osteoconductive scaffold of recombinant peptide based on human collagen type I and β-tri calcium phosphate nanoparticles.
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| Title: | Fabrication and characterization of osteoconductive scaffold of recombinant peptide based on human collagen type I and β-tri calcium phosphate nanoparticles. |
| Other Titles: | ヒトⅠ型コラーゲン様リコンビナントペプチドとβ-リン酸三カルシウムナノ粒子からなる骨形成スキャフォールドの作製および特性評価 |
| Authors: | 降籏, 友和 Browse this author |
| Keywords: | Bone tissue engineering | | rat | | reverse transcription polymerase chain reaction (RT-PCR) |
| Issue Date: | 25-Mar-2019 |
| Publisher: | Hokkaido University |
| Abstract: | Recombinant peptide based on human collagen type I has been introduced as a xeno-free polymer material for various tissue engineering approach. In this study, we fabricated the recombinant human collagen-peptide based scaffold (RCP) applied with β-TCP nanoparticles (RCP-TCP) and assessed the osteoconductive capability of RCP-TCP in cell-culture test and rat bone-forming test. RCP-TCP was prepared by mixing the RCP granules and nanoparticulated β-TCP aqueous dispersion (0, 0.01, 0.1, 1 and 10 wt%). Subsequently, RCP-TCP was characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX), cyto-compatibility testing and real-time RT-PCR. In addition, RCP-TCP was implanted into the defect of rat cranial bone. Radiographic and histological evaluation was carried out at 2 and 4 weeks. In SEM and EDX analyses, RCP-TCP showed β-TCP nanoparticles aggregated on the RCP surface and exhibited the elements of P and Ca. In cell culture tests, RCP-TCP remarkably promoted the proliferation of osteoblastic MC3T3-E1 cell and the expression of osteogenic markers, such as anti-runt-related transcription factor 2, alkaline phosphatase and bone sialoprotein resulted in osteoblastic differentiation. In the rat bone forming test, RCP-TCP significantly stimulated the new bone formation at 2 and 4 weeks, when compared to RCP and no application groups. Therefore, RCP-TCP would be anticipated for application to bone tissue engineering therapy. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第13486号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 教授 田村 正人, 教授 網塚 憲生, 准教授 菅谷 勉 |
| Degree Affiliation: | 歯学研究科(口腔医学専攻) |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/77149 |
| Appears in Collections: | 課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
学位論文 (Theses) > 博士 （歯学）
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