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Effect of thermal control of dry fomites on regulating the survival of human pathogenic bacteria responsible for nosocomial infections

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Title: Effect of thermal control of dry fomites on regulating the survival of human pathogenic bacteria responsible for nosocomial infections
Authors: Shimoda, Tomoko Browse this author →KAKEN DB
Okubo, Torahiko Browse this author →KAKEN DB
Enoeda, Yoshiki Browse this author
Yano, Rika Browse this author →KAKEN DB
Nakamura, Shinji Browse this author →KAKEN DB
Thapa, Jeewan Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Issue Date: 27-Dec-2019
Publisher: PLOS
Journal Title: PLoS ONE
Volume: 14
Issue: 12
Start Page: e0226952
Publisher DOI: 10.1371/journal.pone.0226952
PMID: 31881059
Abstract: We monitored the survival of human pathogenic bacteria [Escherichia coli (ATCC), extended-spectrum beta-lactamase-producing E. coli (Clinical isolate), New Delhi metallo-beta-lactamase-producing E. coli (clinical isolate), Staphylococcus aureus (ATCC)] on dry materials (vinyl chloride, aluminum, plastic, stainless steel) at distinct temperatures (room temperature or 15 degrees C-37 degrees C). These bacteria favored a lower temperature for their prolonged survival on the dry fomites, regardless of the material type. Interestingly, when mixed with S. aureus, E. coli survived for a longer time at a lower temperature. Cardiolipin, which can promote the survival of S. aureus in harsh environments, had no effect on maintaining the survival of E. coli. Although the trends remained unchanged, adjusting the humidity from 40% to 60% affected the survival of bacteria on dry surfaces. Scanning electron microscopic analysis revealed no morphological differences in these bacteria immediately before or after one day of dry conditions. In addition, ATP assessment, a method used to visualize high-touch surfaces in hospitals, was not effective at monitoring bacterial dynamics. A specialized handrail device fitted with a heater, which was maintained at normal human body core temperature, successfully prohibited the prolonged survival of bacteria [Enterococcus faecalis (ATCC), E. coli (ATCC), Pseudomonas aeruginosa (ATCC), S. aureus (ATCC), Acinetobacter baumannii (clinical isolate), and Serratia marcescens (clinical isolate)], with the exception of spore-forming Bacillus subtilis (from our laboratory collection) and the yeast like fungus Candida albicans (from our laboratory collection)] on dry surfaces. Taken together, we concluded that the tested bacteria favor lower temperatures for their survival in dry environments. Therefore, the thermal control of dry fomites has the potential to control bacterial survival on high-touch surfaces in hospitals.
Type: article
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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