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Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique
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Title: | Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique |
Authors: | Miyazaki, Takuji Browse this author | Hanamatsu, Hisatoshi Browse this author →KAKEN DB | Xu, Liang Browse this author | Onodera, Tomohiro Browse this author →KAKEN DB | Furukawa, Jun-ichi Browse this author →KAKEN DB | Homan, Kentaro Browse this author →KAKEN DB | Baba, Rikiya Browse this author | Kawasaki, Toshisuke Browse this author | Iwasaki, Norimasa Browse this author →KAKEN DB |
Keywords: | cartilage damage | osteoarthritis | iPSCs | chondrocytes | GSL-glycome analysis | aminolysis-SALSA | tumorigenicity | glycoconjugates |
Issue Date: | 1-Jan-2020 |
Publisher: | MDPI |
Journal Title: | International Journal of Molecular Sciences |
Volume: | 21 |
Issue: | 1 |
Start Page: | 231 |
Publisher DOI: | 10.3390/ijms21010231 |
Abstract: | Cartilage damage may eventually lead to osteoarthritis because it is difficult to repair. Human-induced pluripotent stem cell (iPSC)-derived chondrocytes may potentially be used to treat cartilage damage, but the tumorigenicity of iPSCs is a major concern for their application in regenerative medicine. Many glycoconjugates serve as stem cell markers, and glycosphingolipids (GSLs) including H type 1 antigen (Fuc alpha 1-2Gal beta 1-3GlcNAc) have been expressed on the surface of iPSCs. The purpose of the present study was to investigate whether GSL-glycome analysis is useful for quality control of residual iPSCs in chondrocytes. We performed GSL-glycome analysis of undifferentiated iPSCs in chondrocytes by combining glycoblotting and aminolysis-sialic acid linkage-specific alkylamidation (SALSA) method, enabling the detection of small quantities of iPSC-specific GSL-glycans from 5 x 10(4) cells. Furthermore, we estimated the residual amount of iPSCs using R-17F antibody, which possesses cytotoxic activity toward iPSCs that is dependent on the Lacto-N-fucopentaose I (LNFP I) of GSL. Moreover, we could detect a small number of LNFP I during mesenchymal stem cells (MSCs) differentiation from iPSCs. This is the first demonstration that GSL-glycome analysis is useful for detecting undifferentiated iPSCs, and can thereby support safe regenerative medicine. |
Rights: | https://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/77500 |
Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc) 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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