Title: | Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease |
Authors: | Nishibata, Yuka Browse this author |
Masuda, Sakiko Browse this author |
Nakazawa, Daigo Browse this author |
Tanaka, Satoshi Browse this author |
Tomaru, Utano Browse this author →KAKEN DB |
Nergui, Mandkhai Browse this author |
Xiao-yu, Jia Browse this author |
Cui, Zhao Browse this author |
Zhao, Ming-hui Browse this author |
Nakabayashi, Kimimasa Browse this author |
Ishizu, Akihiro Browse this author →KAKEN DB |
Keywords: | anti-GBM disease |
type IV collagen |
epitope |
relapse |
protease |
Issue Date: | Apr-2019 |
Publisher: | Elsevier |
Journal Title: | Experimental and Molecular Pathology |
Volume: | 107 |
Start Page: | 165 |
End Page: | 170 |
Publisher DOI: | 10.1016/j.yexmp.2019.02.005 |
Abstract: | The major epitopes recognized by autoantibodies in anti-glomerular basement membrane (GBM) disease are found in the α3-subunit non-collagenous domain of type IV collagen [α3(IV)NC1], which is present in the glomerular and alveolar basement membranes. These epitopes are structurally cryptic, owing to the hexamer formation of the non-collagenous domain of α3, α4, and α5 subunits and are expressed by the dissociation of the hexamer. Anti-GBM disease usually manifests as a single attack (SA), and we rarely see patients who repeatedly relapse. We recently treated a patient with anti-GBM disease who exhibited repeated relapse (RR). Here, we conducted immunohistochemistry of formalin-fixed paraffin-embedded normal kidney sections and immunoblotting using recombinant human α3(IV)NC1 to compare the epitopes recognized by anti-GBM antibodies in the RR patient and SA patients. Although a clear staining of GBM especially in the connecting basement membrane of Bowman’s capsule was observed when IgGs of SA patients were used as primary antibodies, such staining was not obtained when IgG of the RR patient was employed. In immunoblotting of α3(IV)NC1 using the IgG of the RR patient as a primary antibody, an 18-kDa band was detected besides the 56.8-kDa band corresponding to the whole-size α3(IV)NC1. Whereas the 56.8-kDa band disappeared after digestion of the recombinant α3(IV)NC1 by protease, the 18-kDa band remained. Furthermore, the 18-kDa band was not detected by a commercially available anti-α3(IV)NC1 monoclonal antibody. These findings suggest that the IgG of the RR patient recognizes the epitope distinct from that recognized by the anti-α3(IV)NC1 monoclonal antibody. |
Rights: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/77759 |
Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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