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Detection of early adenocarcinoma of the esophagogastric junction by spraying an enzyme-activatable fluorescent probe targeting Dipeptidyl peptidase-IV

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Title: Detection of early adenocarcinoma of the esophagogastric junction by spraying an enzyme-activatable fluorescent probe targeting Dipeptidyl peptidase-IV
Authors: Yamamoto, Keikokw Browse this author
Ohnishi, Shunsuke Browse this author →KAKEN DB
Mizushima, Takeshi Browse this author
Kodaira, Junichi Browse this author
Ono, Masayoshi Browse this author
Hatanaka, Yutaka Browse this author →KAKEN DB
Hatanaka, Kanako C. Browse this author
Kuriki, Yugo Browse this author
Kamiya, Mako Browse this author →KAKEN DB
Ehira, Nobuyuki Browse this author
Shinada, Keisuke Browse this author
Takahashi, Hiroaki Browse this author
Shimizu, Yuichi Browse this author →KAKEN DB
Urano, Yasuteru Browse this author
Sakamoto, Naoya Browse this author →KAKEN DB
Keywords: Fluorescence imaging
Dipeptidyl peptidase-IV
Adenocarcinoma of the esophagogastric junction
Issue Date: 28-Jan-2020
Publisher: BioMed Central
Journal Title: BMC cancer
Volume: 20
Issue: 1
Start Page: 64
Publisher DOI: 10.1186/s12885-020-6537-9
Abstract: Background It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7, 85.7, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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