| Title: | Detection of early adenocarcinoma of the esophagogastric junction by spraying an enzyme-activatable fluorescent probe targeting Dipeptidyl peptidase-IV |
| Authors: | Yamamoto, Keikokw Browse this author |
| Ohnishi, Shunsuke Browse this author →KAKEN DB |
| Mizushima, Takeshi Browse this author |
| Kodaira, Junichi Browse this author |
| Ono, Masayoshi Browse this author |
| Hatanaka, Yutaka Browse this author →KAKEN DB |
| Hatanaka, Kanako C. Browse this author |
| Kuriki, Yugo Browse this author |
| Kamiya, Mako Browse this author →KAKEN DB |
| Ehira, Nobuyuki Browse this author |
| Shinada, Keisuke Browse this author |
| Takahashi, Hiroaki Browse this author |
| Shimizu, Yuichi Browse this author →KAKEN DB |
| Urano, Yasuteru Browse this author |
| Sakamoto, Naoya Browse this author →KAKEN DB |
| Keywords: | Fluorescence imaging |
| Dipeptidyl peptidase-IV |
| Adenocarcinoma of the esophagogastric junction |
| Issue Date: | 28-Jan-2020 |
| Publisher: | BioMed Central |
| Journal Title: | BMC cancer |
| Volume: | 20 |
| Issue: | 1 |
| Start Page: | 64 |
| Publisher DOI: | 10.1186/s12885-020-6537-9 |
| Abstract: | Background It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7, 85.7, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/77824 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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