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Genetic and Biological Diversity of Porcine Sapeloviruses Prevailing in Zambia

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Title: Genetic and Biological Diversity of Porcine Sapeloviruses Prevailing in Zambia
Authors: Harima, Hayato Browse this author
Kajihara, Masahiro Browse this author
Simulundu, Edgar Browse this author
Bwalya, Eugene Browse this author
Qiu, Yongjin Browse this author
Isono, Mao Browse this author
Okuya, Kosuke Browse this author
Gonzalez, Gabriel Browse this author
Yamagishi, Junya Browse this author
Hang'ombe, Bernard M. Browse this author
Sawa, Hirofumi Browse this author
Mweene, Aaron S. Browse this author
Takada, Ayato Browse this author →KAKEN DB
Keywords: porcine sapelovirus
prevalence
complete genome
characterization
recombination
Zambia
Issue Date: Feb-2020
Publisher: MDPI
Journal Title: Viruses-Basel
Volume: 12
Issue: 2
Start Page: 180
Publisher DOI: 10.3390/v12020180
Abstract: Porcine sapelovirus (PSV) has been detected worldwide in pig populations. Although PSV causes various symptoms such as encephalomyelitis, diarrhea, and pneumonia in pigs, the economic impact of PSV infection remains to be determined. However, information on the distribution and genetic diversity of PSV is quite limited, particularly in Africa. In this study, we investigated the prevalence of PSV infection in Zambia and characterized the isolated PSVs genetically and biologically. We screened 147 fecal samples collected in 2018 and found that the prevalences of PSV infection in suckling pigs and fattening pigs were high (36.2% and 94.0%, respectively). Phylogenetic analyses revealed that the Zambian PSVs were divided into three different lineages (Lineages 1-3) in the clade consisting of Chinese strains. The Zambian PSVs belonging to Lineages 2 and 3 replicated more efficiently than those belonging to Lineage 1 in Vero E6 and BHK cells. Bioinformatic analyses revealed that genetic recombination events had occurred and the recombination breakpoints were located in the L and 2A genes. Our results indicated that at least two biologically distinct PSVs could be circulating in the Zambian pig population and that genetic recombination played a role in the evolution of PSVs.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/78074
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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