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Development of a High-Throughput Serum Neutralization Test Using Recombinant Pestiviruses Possessing a Small Reporter Tag
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| Title: | Development of a High-Throughput Serum Neutralization Test Using Recombinant Pestiviruses Possessing a Small Reporter Tag |
| Authors: | Tetsuo, Madoka Browse this author | | Matsuno, Keita Browse this author →KAKEN DB | | Tamura, Tomokazu Browse this author | | Fukuhara, Takasuke Browse this author →KAKEN DB | | Kim, Taksoo Browse this author | | Okamatsu, Masatoshi Browse this author →KAKEN DB | | Tautz, Norbert Browse this author | | Matsuura, Yoshiharu Browse this author →KAKEN DB | | Sakoda, Yoshihiro Browse this author →KAKEN DB |
| Keywords: | border disease | | bovine viral diarrhea | | classical swine fever | | pestivirus | | serum neutralization test | | reporter virus |
| Issue Date: | Mar-2020 |
| Publisher: | MDPI |
| Journal Title: | Pathogens |
| Volume: | 9 |
| Issue: | 3 |
| Start Page: | 188 |
| Publisher DOI: | 10.3390/pathogens9030188 |
| Abstract: | A serum neutralization test (SNT) is an essential method for the serological diagnosis of pestivirus infections, including classical swine fever, because of the cross reactivity of antibodies against pestiviruses and the non-quantitative properties of antibodies in an enzyme-linked immunosorbent assay. In conventional SNTs, an immunoperoxidase assay or observation of cytopathic effect after incubation for 3 to 7 days is needed to determine the SNT titer, which requires labor-intensive or time-consuming procedures. Therefore, a new SNT, based on the luciferase system and using classical swine fever virus, bovine viral diarrhea virus, and border disease virus possessing the 11-amino-acid subunit derived from NanoLuc luciferase was developed and evaluated; this approach enabled the rapid and easy determination of the SNT titer using a luminometer. In the new method, SNT titers can be determined tentatively at 2 days post-infection (dpi) and are comparable to those obtained by conventional SNTs at 3 or 4 dpi. In conclusion, the luciferase-based SNT can replace conventional SNTs as a high-throughput antibody test for pestivirus infections. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/78080 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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