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Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter

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Title: Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter
Other Titles: 野生エゾシカにおけるルーメン細菌叢の季節変動ならびに冬季の繊維消化に関わる未知細菌群の特定
Authors: Yamano, Hidehisa1 Browse this author
Ichimura, Yasuhiro2 Browse this author
Sawabe, Yoshihiko3 Browse this author
Koike, Satoshi4 Browse this author →KAKEN DB
Suzuki, Yutaka5 Browse this author →KAKEN DB
Kobayashi, Yasuo6 Browse this author →KAKEN DB
Authors(alt): 山野秀尚1
市村, 康裕2
沢辺, 佳彦3
小池, 聡4
鈴木, 裕5
小林, 泰男6
Keywords: ecology
fiber digestion
quantitation
rumen bacteria
sika deer
Issue Date: Jun-2019
Publisher: John Wiley & Sons
Journal Title: Animal science journal
Volume: 90
Issue: 6
Start Page: 790
End Page: 798
Publisher DOI: 10.1111/asj.13203
PMID: 30983093
Abstract: Rumen digesta was obtained from wild Hokkaido sika deer to compare bacterial flora between summer and winter. Bacterial flora was characterized with molecular-based approaches and enrichment cultivation. Bacteroidetes was shown as a major phylum followed by Firmicutes, with similar proportions in both seasons. However, two phylogenetically unique groups in Bacteroidetes were found in each season: unknown group A in winter and unknown group B in summer. The ruminal abundance of unknown group A was the highest followed by Ruminococcus flavefaciens in winter. Moreover, the abundance of these two was higher in winter than in summer. In contrast, the abundance of unknown group B was higher in summer than in winter. In addition, this group showed the highest abundance in summer among the bacteria quantified. Unknown group A was successfully enriched by cultivating with oak bark and sterilized rumen fluid, particularly that from deer. Bacteria of this group were distributed in association with the solid rather than the liquid rumen fraction, and were detected as small cocci. Accordingly, unknown group A is assumed to be involved in degradation of fibrous materials. These results suggest that wild Hokkaido sika deer develop a rumen bacterial flora in response to changes in dietary conditions.
北海道知床半島ウトロ高原に生息する野生エゾシカ計20頭のルーメン細菌叢について、冬と夏で比較した。BacteroidetesとFirmicutesが両季節で優勢であったが、Bacteroidetesには季節特有の未知細菌群が見られ、各々未知菌群A(冬)および同B(夏)と命名した。両者の定量系を確立し、主要既知菌種(8種)とともに菌叢構成を調べたところ、冬には未知菌群Aが代表的な繊維分解菌Ruminococcus flavefaciens を量的に上回り、夏には未知菌群Bが定量した菌の中で最大値を示した。したがって、両未知菌群の増減が季節的な食餌条件の変化にともなうルーメン菌叢の反応であると解された。定量PCR法とFISH法により未知菌群Aの動態について解析したところ、ミズナラ樹皮粉末と滅菌ルーメン液上清(とくにエゾシカのルーメン液上清)で本菌群の集積が促進されること、また本未知菌群Aはルーメン内では液相よりも固相により多く分布することが明らかになった。これらの結果から、未知菌群Aは冬の主要食餌である樹皮などの植物繊維の消化に関与している可能性が示唆された。
Rights: This is the peer reviewed version of the following article: "Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter", Animal Science Journal 90(6) 790-798,June 2019, which has been published in final form at https://doi.org/10.1111/asj.13203. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
URI: http://hdl.handle.net/2115/78283
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 泰男

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