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Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel

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Title: Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel
Authors: Hontani, Kazutoshi Browse this author →KAKEN DB
Onodera, Tomohiro Browse this author →KAKEN DB
Terashima, Michiyo Browse this author
Momma, Daisuke Browse this author →KAKEN DB
Matsuoka, Masatake Browse this author →KAKEN DB
Baba, Rikiya Browse this author →KAKEN DB
Joutoku, Zenta Browse this author
Matsubara, Shinji Browse this author
Homan, Kentaro Browse this author →KAKEN DB
Hishimura, Ryosuke Browse this author
Xu, Liang Browse this author
Iwasaki, Norimasa Browse this author →KAKEN DB
Keywords: Cartilage repair
iPS cells
Mesenchymal stem cells
Alginate gel
Chondrogenic differentiation
Issue Date: May-2019
Publisher: John Wiley & Sons
Journal Title: Journal of biomedical materials research part A
Volume: 107
Issue: 5
Start Page: 1086
End Page: 1093
Publisher DOI: 10.1002/jbm.a.36615
Abstract: As articular cartilages have rarely healed by themselves because of their characteristics of avascularity and low cell density, surgical intervention is ideal for patients with cartilaginous injuries. Because of structural characteristics of the cartilage tissue, a three-dimensional culture of stem cells in biomaterials is a favorable system on cartilage tissue engineering. Induced pluripotent stem cells (iPSCs) are a new cell source in cartilage tissue engineering for its characteristics of self-renewal capability and pluripotency. However, the optimal cultivation condition for chondrogenesis of iPSCs is still unknown. Here we show that a novel chondrogenic differentiation method of iPSCs using the combination of three-dimensional cultivation in ultra-purified alginate gel (UPAL gel) and multi-step differentiation via mesenchymal stem cell-like cells (iPS-MSCs) could efficiently and specifically differentiate iPSCs into chondrocytes. The iPS-MSCs in UPAL gel culture sequentially enhanced the expression of chondrogenic marker without the upregulation of that of osteogenic and adipogenic marker and histologically showed homogeneous chondrogenic extracellular matrix formation. Our results suggest that the pluripotency of iPSCs can be controlled when iPSCs are differentiated into iPS-MSCs before embedding in UPAL gel. These results lead to the establishment of an efficient three-dimensional system to engineer artificial cartilage tissue from iPSCs for cartilage regeneration. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1086-1093, 2019.
Rights: This is the peer reviewed version of the following article: Kazutoshi Hontani, Tomohiro Onodera, Michiyo Terashima, Daisuke Momma, Masatake Matsuoka Rikiya Baba, Zenta Joutoku, Shinji Matsubara, Kentaro Homan, Ryosuke Hishimura, Liang Xu, Norimasa Iwasaki (2018) Chondrogenic differentiation of mouse induced pluripotent stem cells using the three‐dimensional culture with ultra‐purified alginate gel. J. Biomed. Mater. Res. Part A, 88: 1086–1093, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小野寺 智洋

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