| Title: | Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel |
| Authors: | Hontani, Kazutoshi Browse this author →KAKEN DB |
| Onodera, Tomohiro Browse this author →KAKEN DB |
| Terashima, Michiyo Browse this author |
| Momma, Daisuke Browse this author →KAKEN DB |
| Matsuoka, Masatake Browse this author →KAKEN DB |
| Baba, Rikiya Browse this author →KAKEN DB |
| Joutoku, Zenta Browse this author |
| Matsubara, Shinji Browse this author |
| Homan, Kentaro Browse this author →KAKEN DB |
| Hishimura, Ryosuke Browse this author |
| Xu, Liang Browse this author |
| Iwasaki, Norimasa Browse this author →KAKEN DB |
| Keywords: | Cartilage repair |
| iPS cells |
| Mesenchymal stem cells |
| Alginate gel |
| Chondrogenic differentiation |
| Issue Date: | May-2019 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Journal of biomedical materials research part A |
| Volume: | 107 |
| Issue: | 5 |
| Start Page: | 1086 |
| End Page: | 1093 |
| Publisher DOI: | 10.1002/jbm.a.36615 |
| Abstract: | As articular cartilages have rarely healed by themselves because of their characteristics of avascularity and low cell density, surgical intervention is ideal for patients with cartilaginous injuries. Because of structural characteristics of the cartilage tissue, a three-dimensional culture of stem cells in biomaterials is a favorable system on cartilage tissue engineering. Induced pluripotent stem cells (iPSCs) are a new cell source in cartilage tissue engineering for its characteristics of self-renewal capability and pluripotency. However, the optimal cultivation condition for chondrogenesis of iPSCs is still unknown. Here we show that a novel chondrogenic differentiation method of iPSCs using the combination of three-dimensional cultivation in ultra-purified alginate gel (UPAL gel) and multi-step differentiation via mesenchymal stem cell-like cells (iPS-MSCs) could efficiently and specifically differentiate iPSCs into chondrocytes. The iPS-MSCs in UPAL gel culture sequentially enhanced the expression of chondrogenic marker without the upregulation of that of osteogenic and adipogenic marker and histologically showed homogeneous chondrogenic extracellular matrix formation. Our results suggest that the pluripotency of iPSCs can be controlled when iPSCs are differentiated into iPS-MSCs before embedding in UPAL gel. These results lead to the establishment of an efficient three-dimensional system to engineer artificial cartilage tissue from iPSCs for cartilage regeneration. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1086-1093, 2019. |
| Rights: | This is the peer reviewed version of the following article: Kazutoshi Hontani, Tomohiro Onodera, Michiyo Terashima, Daisuke Momma, Masatake Matsuoka Rikiya Baba, Zenta Joutoku, Shinji Matsubara, Kentaro Homan, Ryosuke Hishimura, Liang Xu, Norimasa Iwasaki (2018) Chondrogenic differentiation of mouse induced pluripotent stem cells using the three‐dimensional culture with ultra‐purified alginate gel. J. Biomed. Mater. Res. Part A, 88: 1086–1093, which has been published in final form at https://doi.org/10.1002/jbm.a.36615. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/78388 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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