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The effect of alkaline pretreatment on the biochemical characteristics and fibril-forming abilities of types I and II collagen extracted from bester sturgeon by-products
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| Title: | The effect of alkaline pretreatment on the biochemical characteristics and fibril-forming abilities of types I and II collagen extracted from bester sturgeon by-products |
| Authors: | Meng, Dawei Browse this author | | Tanaka, Hiroyuki Browse this author →KAKEN DB | | Kobayashi, Taishi Browse this author | | Hatayama, Hirosuke Browse this author | | Zhang, Xi Browse this author | | Ura, Kazuhiro Browse this author →KAKEN DB | | Yunoki, Shunji Browse this author | | Takagi, Yasuaki Browse this author →KAKEN DB |
| Keywords: | Alkaline pretreatment | | Type I collagen | | Type II collagen | | Collagen fibril | | Thermal stability | | By-products |
| Issue Date: | 15-Jun-2019 |
| Journal Title: | International Journal of Biological Macromolecules |
| Volume: | 131 |
| Start Page: | 572 |
| End Page: | 580 |
| Publisher DOI: | 10.1016/j.ijbiomac.2019.03.091 |
| Abstract: | Non-mammalian collagens have attracted increasing attention for industrial and biomedical use. We have therefore evaluated extraction conditions and the biochemical properties of collagens from aquacultured sturgeon. Pepsin-soluble type I and type II collagen were respectively extracted from the skin and notochord of bester sturgeon by-products, with yields of 63.9 ± 0.19% and 35.5 ± 0.68%. Collagen extraction efficiency was improved by an alkaline pretreatment of the skin and notochord (fewer extraction cycles were required), but the final yields decreased to 56.2 ± 0.84% for type I and 31.8 ± 1.13% for type II. Alkaline pretreatment did not affect the thermal stability or triple-helical structure of both types of collagen. Types I and II collagen formed re-assembled fibril structures in vitro, under different conditions. Alkaline pretreatment slowed down the formation of type I collagen fibrils and specifically inhibited the formation of thick fibril-bundle structures. In contrast, alkaline pretreatment did not change type II collagen fibril formation. In conclusion, alkaline pretreatment of sturgeon skin and notochord is an effective method to accelerate collagen extraction process of types I and II collagen without changing their biochemical properties. However, it decreases the yield of both collagens and specifically changes the fibril-forming ability of type I collagen. |
| Rights: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/78566 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 都木 靖彰
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