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Function of leukaemia inhibitory factor in spermatogenesis of a teleost fish, the medaka Oryzias latipes
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Title: | Function of leukaemia inhibitory factor in spermatogenesis of a teleost fish, the medaka Oryzias latipes |
Authors: | Satoh, Ryuichi Browse this author | Bando, Hisanori Browse this author | Sakai, Noriyoshi Browse this author | Kotani, Tomoya Browse this author | Yamashita, Masakane Browse this author →KAKEN DB |
Keywords: | Cell culture | Lif expression | Mitosis | Spermatogenesis | Teleost testis |
Issue Date: | Dec-2019 |
Publisher: | Cambridge University Press |
Journal Title: | Zygote |
Volume: | 27 |
Issue: | 6 |
Start Page: | 423 |
End Page: | 431 |
Publisher DOI: | 10.1017/S0967199419000558 |
Abstract: | In response to gonadotropins and androgens, testicular cells produce various molecules that control proper proliferation and differentiation of spermatogenic cells through their paracrine and autocrine actions. However, molecules functioning downstream of the hormonal stimulation are poorly understood. Leukaemia inhibitory factor (Lif) is known to maintain the pluri-potency of stem cells including embryonic stem cells and primordial germ cells at least in vitro, but its actual roles in vivo remain to be elucidated. To clarify the function of Lif in teleost (medaka) testes, we examined the effects of Lif on spermatogenesis in a newly established cell culture system using a cell line (named Mtp1) derived from medaka testicular somatic cells as feeder cells. We found that addition of baculovirus-produced recombinant medaka Lif to the culture medium or co-culture with Lif-overexpressing Mtp1 cells increased the number of spermatogonia. In situ hybridization and immunohistochemical analyses of the medaka testes showed that mRNAs and proteins of Lif are expressed in spermatogonia and the surrounding Sertoli cells, with higher expression levels in type A (undifferentiated) spermatogonia than in type B (differentiated) spermatogonia. Our findings suggest that Lif regulates spermatogonial cell proliferation in the medaka. |
Rights: | This article has been published in a revised form in Zygote [https://doi.org/10.1017/S0967199419000558]. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. © Cambridge University Press 2019. | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/78759 |
Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山下 正兼
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