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Function of leukaemia inhibitory factor in spermatogenesis of a teleost fish, the medaka Oryzias latipes

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Title: Function of leukaemia inhibitory factor in spermatogenesis of a teleost fish, the medaka Oryzias latipes
Authors: Satoh, Ryuichi Browse this author
Bando, Hisanori Browse this author
Sakai, Noriyoshi Browse this author
Kotani, Tomoya Browse this author
Yamashita, Masakane Browse this author →KAKEN DB
Keywords: Cell culture
Lif expression
Mitosis
Spermatogenesis
Teleost testis
Issue Date: Dec-2019
Publisher: Cambridge University Press
Journal Title: Zygote
Volume: 27
Issue: 6
Start Page: 423
End Page: 431
Publisher DOI: 10.1017/S0967199419000558
Abstract: In response to gonadotropins and androgens, testicular cells produce various molecules that control proper proliferation and differentiation of spermatogenic cells through their paracrine and autocrine actions. However, molecules functioning downstream of the hormonal stimulation are poorly understood. Leukaemia inhibitory factor (Lif) is known to maintain the pluri-potency of stem cells including embryonic stem cells and primordial germ cells at least in vitro, but its actual roles in vivo remain to be elucidated. To clarify the function of Lif in teleost (medaka) testes, we examined the effects of Lif on spermatogenesis in a newly established cell culture system using a cell line (named Mtp1) derived from medaka testicular somatic cells as feeder cells. We found that addition of baculovirus-produced recombinant medaka Lif to the culture medium or co-culture with Lif-overexpressing Mtp1 cells increased the number of spermatogonia. In situ hybridization and immunohistochemical analyses of the medaka testes showed that mRNAs and proteins of Lif are expressed in spermatogonia and the surrounding Sertoli cells, with higher expression levels in type A (undifferentiated) spermatogonia than in type B (differentiated) spermatogonia. Our findings suggest that Lif regulates spermatogonial cell proliferation in the medaka.
Rights: This article has been published in a revised form in Zygote [https://doi.org/10.1017/S0967199419000558]. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. © Cambridge University Press 2019.
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/78759
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山下 正兼

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