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A novel sponge-derived protein thrombocorticin is a new agonist for thrombopoietin receptor

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Title: A novel sponge-derived protein thrombocorticin is a new agonist for thrombopoietin receptor
Authors: Watari, Hiromi Browse this author
Nakajima, Hiroya Browse this author
Atsuumi, Wataru Browse this author
Nakamura, Takanori Browse this author
Nanya, Takeshi Browse this author
Ise, Yuji Browse this author
Sakai, Ryuichi Browse this author →KAKEN DB
Keywords: Hematopoietic cytokine
Marine sponge
Issue Date: Jul-2019
Publisher: Elsevier
Journal Title: Comparative biochemistry and physiology Part C : Toxicology & pharmacology
Volume: 221
Start Page: 82
End Page: 88
Publisher DOI: 10.1016/j.cbpc.2019.04.003
Abstract: We screened 868 marine extracts in search of hematopoietic molecules resulted in findings of several extracts that proliferated Ba/F3-HuMpl cells but not the cells expressed with other hematopoietic cytokine receptors, EPO and G-CSF. Separation of the most potent extract of a Micronesian sponge Corticium sp., guided by the cell proliferation assay using Ba/F3-HuMpl cells resulted in an isolation of thrombocorticin (ThC), a novel 14 kDa protein as an active principal. ThC displayed concentration-dependent proliferation of Ba/F3-HuMpl cells, and had a stronger activity than that of eltrombopag, a small molecule drug used to treat thrombocytopenia. ThC induced phosphorylation of STAT5, suggesting that it activates Jak/STAT pathway as in the case of TPO. These results together indicated that ThC is a specific agonist for c-Mpl, although the size and shape differs largely from TPO. Here we present isolation, characterization and biological activity of ThC.
Rights: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 酒井 隆一

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