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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
A novel sponge-derived protein thrombocorticin is a new agonist for thrombopoietin receptor
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| Title: | A novel sponge-derived protein thrombocorticin is a new agonist for thrombopoietin receptor |
| Authors: | Watari, Hiromi Browse this author | | Nakajima, Hiroya Browse this author | | Atsuumi, Wataru Browse this author | | Nakamura, Takanori Browse this author | | Nanya, Takeshi Browse this author | | Ise, Yuji Browse this author | | Sakai, Ryuichi Browse this author →KAKEN DB |
| Keywords: | Hematopoietic cytokine | | Thrombopoietin | | Agonist | | Marine sponge | | Protein |
| Issue Date: | Jul-2019 |
| Publisher: | Elsevier |
| Journal Title: | Comparative biochemistry and physiology Part C : Toxicology & pharmacology |
| Volume: | 221 |
| Start Page: | 82 |
| End Page: | 88 |
| Publisher DOI: | 10.1016/j.cbpc.2019.04.003 |
| Abstract: | We screened 868 marine extracts in search of hematopoietic molecules resulted in findings of several extracts that proliferated Ba/F3-HuMpl cells but not the cells expressed with other hematopoietic cytokine receptors, EPO and G-CSF. Separation of the most potent extract of a Micronesian sponge Corticium sp., guided by the cell proliferation assay using Ba/F3-HuMpl cells resulted in an isolation of thrombocorticin (ThC), a novel 14 kDa protein as an active principal. ThC displayed concentration-dependent proliferation of Ba/F3-HuMpl cells, and had a stronger activity than that of eltrombopag, a small molecule drug used to treat thrombocytopenia. ThC induced phosphorylation of STAT5, suggesting that it activates Jak/STAT pathway as in the case of TPO. These results together indicated that ThC is a specific agonist for c-Mpl, although the size and shape differs largely from TPO. Here we present isolation, characterization and biological activity of ThC. |
| Rights: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/78805 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 酒井 隆一
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