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Enzymatic production of xylooligosaccharides from red alga dulse (Palmaria sp.) wasted in Japan

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Title: Enzymatic production of xylooligosaccharides from red alga dulse (Palmaria sp.) wasted in Japan
Authors: Yamamoto, Yohei Browse this author
Kishimura, Hideki Browse this author →KAKEN DB
Kinoshita, Yasunori Browse this author
Saburi, Wataru Browse this author →KAKEN DB
Kumagai, Yuya Browse this author →KAKEN DB
Yasui, Hajime Browse this author →KAKEN DB
Ojima, Takao Browse this author →KAKEN DB
Keywords: Xylooligosaccharides
Red alga
Palmaria sp.
Xylan
Hemicellulase
Issue Date: Jul-2019
Publisher: Elsevier
Journal Title: Process biochemistry
Volume: 82
Start Page: 117
End Page: 122
Publisher DOI: 10.1016/j.procbio.2019.03.030
Abstract: Red alga dulse (Palmaria sp.) has xylan with a small amount of cellulose and less lignin, hence these characteristics may provide some advantages in the enzymatic production of xylooligosaccharides (XOS). To evaluate dulse xylan as XOS source, we prepared xylan rich fraction (DXRF) and attempted XOS production using commercial enzymes. 13.8% of xylan rich fraction (DXRF: containing 52.2% of xylan) was obtained from dried dulse powder. We then evaluated the hydrolysis products of two commercial enzymes, revealing that hydrolysate of Hemicellulase amano 90 contained less xylose. The effective XOS production by the enzyme was evaluated in terms of enzyme dose, DXRF concentration and reaction period, showing that 66.6% of XOS was obtained from DXRF with the hydrolysis rate of 82% under the following condition: 54 U Hemicellulase amano 90, 10 mg/ml DXRF, pH 4.5, 50 degrees C for 24 h. These results indicated that red algae xylan is suitable for XOS source.
Rights: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/78807
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸村 栄毅

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